1dz9

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[[Image:1dz9.gif|left|200px]]<br /><applet load="1dz9" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1dz9.gif|left|200px]]
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caption="1dz9, resolution 1.9&Aring;" />
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'''PUTATIVE OXO COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA'''<br />
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{{Structure
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|PDB= 1dz9 |SIZE=350|CAPTION= <scene name='initialview01'>1dz9</scene>, resolution 1.9&Aring;
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|SITE= <scene name='pdbsite=K1:This+Is+The+Intramolecular+K+Binding+Site+For+Monomer+A+...'>K1</scene>, <scene name='pdbsite=K2:This+Is+The+Intramolecular+K+Binding+Site+For+Monomer+B+...'>K2</scene>, <scene name='pdbsite=K3:Intermolecular+K+Site'>K3</scene> and <scene name='pdbsite=OXO:O'>OXO</scene>
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|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>, <scene name='pdbligand=CAM:CAMPHOR'>CAM</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''PUTATIVE OXO COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1DZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] with <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=HEM:'>HEM</scene>, <scene name='pdbligand=O:'>O</scene>, <scene name='pdbligand=CAM:'>CAM</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=K1:This+Is+The+Intramolecular+K+Binding+Site+For+Monomer+A+...'>K1</scene>, <scene name='pdbsite=K2:This+Is+The+Intramolecular+K+Binding+Site+For+Monomer+B+...'>K2</scene>, <scene name='pdbsite=K3:Intermolecular+K+Site'>K3</scene> and <scene name='pdbsite=OXO:O'>OXO</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DZ9 OCA].
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1DZ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DZ9 OCA].
==Reference==
==Reference==
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The catalytic pathway of cytochrome p450cam at atomic resolution., Schlichting I, Berendzen J, Chu K, Stock AM, Maves SA, Benson DE, Sweet RM, Ringe D, Petsko GA, Sligar SG, Science. 2000 Mar 3;287(5458):1615-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10698731 10698731]
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The catalytic pathway of cytochrome p450cam at atomic resolution., Schlichting I, Berendzen J, Chu K, Stock AM, Maves SA, Benson DE, Sweet RM, Ringe D, Petsko GA, Sligar SG, Science. 2000 Mar 3;287(5458):1615-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10698731 10698731]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: reaction intermediate]]
[[Category: reaction intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:22:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:46:51 2008''

Revision as of 08:46, 20 March 2008

Image:1dz9.gif


PDB ID 1dz9

Drag the structure with the mouse to rotate
, resolution 1.9Å
Sites: , , and
Ligands: , , , and
Coordinates: save as pdb, mmCIF, xml



PUTATIVE OXO COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA


Overview

Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature-a reaction that requires high temperature to proceed in the absence of a catalyst. Structures were obtained for three intermediates in the hydroxylation reaction of camphor by P450cam with trapping techniques and cryocrystallography. The structure of the ferrous dioxygen adduct of P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to an intermediate that would be consistent with an oxyferryl species. The structures show conformational changes in several important residues and reveal a network of bound water molecules that may provide the protons needed for the reaction.

About this Structure

1DZ9 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

The catalytic pathway of cytochrome p450cam at atomic resolution., Schlichting I, Berendzen J, Chu K, Stock AM, Maves SA, Benson DE, Sweet RM, Ringe D, Petsko GA, Sligar SG, Science. 2000 Mar 3;287(5458):1615-22. PMID:10698731

Page seeded by OCA on Thu Mar 20 10:46:51 2008

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