Kinesin

From Proteopedia

Revision as of 11:46, 1 September 2014

Template:STRUCTURE 2zfi

3D Structure of Kinesin
The image is a Crystal Structure of the Kif1A Motor Domain Before Mg Release (2zfi)

Kinesin

Kinesins are eukaryotic motor proteins which move along microtubules. Kinesin (KIF) is a dimer consisting of 2 heavy chains and two light chains. The heavy chain contains the globular motor domain (MD), flexible neck linker (FNL) and a long coiled-coil stalk which intertwines to form the dimer. The light chain (KLC) forms the tail region. The KLC contains a cargo binding domain which is called TPR (Tetratricopeptide repeat). The KIFs are named by their gene number. KIF contains a forkhead-associated domain (FHA) which is involved in phosphopeptide recognition. Kinesin protein in yeast is called Kar3. Eg5 or KIF11 is a kinesin (Kinesin-5) which participates in mitosis. KCBP is a Kinesin-like Calmodulin Binding Protein. NOD is a Drosophila chromosome-associated kinesin.

3D Structures of Kinesin

Updated on 01-September-2014

Kinesin heavy chain (KIF)

2zfi, 2zfj, 2zfk, 2zfl, 2zfm – mKIF1A – mouse
2vvg – KIF2 MD – Giardia intestinalis
2g1l - hKIF1C FHA domain
2owm – NcKIF1C MD – Neurospora crassa
1goj – NcKIF MD
3bfn – hKIF22 MD
3b6u, 3b6v – hKIF3B MD
2rep – hKIFC1 MD
2eh0 - hKIF1B FHA domain – NMR
2edu – hKIF22 C-terminal – NMR
2v14 – hKIF16B
2heh – hKIF2C
2cow – hKIF13B CAP-gly domain – NMR
1bg2 – hKIF MD
3kin, 2kin – rKIF MD - rat
1ry6 – KIF MD – Plasmodium falciparum
2ncd, 1cz7 – DmKIF MD non-claret disjuctional (NCD) dimer – Drosophila melanogaster
2y5w, 2y65 - DmKIF MD

Kinesin heavy chain complex with nucleotide

3gbj – hKIF13B MD+ADP – human
1vfv, 1vfw – mKIF1A MD+Mg-AMPPNP
1vfx - mKIF1A MD+ADP-Mg-AlFx
1vfz - mKIF1A MD+ADP-Mg-VO4
1v8j – mKIF2C MD+Mg-ADP
1i5s - mKIF2C MD (mutant)+Mg-ADP
1i6i - mKIF2C MD (mutant)+Mg-AMPPCP
1v8k - mKIF2C MD+Mg-AMPPNP
3zfc, 3zfd – mKIF4 MD+Mg-AMPPNP
2cjo – hKIF13+inhibitor 30
2nr8 – hKIF9+ADP
2h58 – hKIFC3+ADP
2gry – hKIF2+ADP
2hfx – pKIF1A head+AMPPNP – pig
2hxh - pKIF1A head+ADP

Kinesin heavy chain complex with protein

2wbe – cKIF MD+FNL+Tubulin A+Tubulin B+AMPPNP – cow
1ia0 – KIF1A MD+Tubulin A+Tubulin B+ATP - pig
3edl – cKIF MD+ Tubulin A+Tubulin B – EM
4hna – hKIF1 + Tubulin A + Tubulin B + designed ankyrin repeat protein + Mg-ADP + GDP + GTP + AlF4
2p4n – hKIF+ Tubulin A+Tubulin B – EM docking
1mkj – hKIF MD+docked FNL
1n6m – DmKIF MD+FNL
4atx - rKIF MD+ Tubulin A+Tubulin B – EM

Kinesin light chain (KLC)

3edt – hKLC2
3ceq, 3nf1 – hKLC2 TPR domain
3zfw - mKLC2 TPR domain + peptide

Kinesin-5 motor domain

4b7b - hKIF11 (mutant) + ADP + Co + Cd
3ken, 2wog, 2x2r, 2xae, 4bbg - hKIF11 +S-trityl-L-cysteine derivative + ADP + Mg
2uyi, 2uym, 2gm1, 2pg2, 2fme, 2x7d, 2x7c, 2ieh, 3cjo, 2g1q, 2fl2, 2fl6, 1yrs, 2q2y, 2fky, 2q2z, 3k3b, 2x7e, 4a50, 4a51, 3zcw - hKIF11+inhibitor +ADP + Mg
4bxn - hKIF11+inhibitor +ADP + Cd
3l9h - hKIF11+inhibitor +ADP
1x88, 1q0b - hKIF11 +Mg + ADP+monastrol
4ap0, 4a5y - hKIF11 +MgADP + ispinesib + Mg
3hqd - hKIF11 +AMPPNP+Mg
1ii6, 4a1z, 4a28 - hKIF11 +ADP+Mg
3k5e - hKIF11 +ADP+enastrol + Mg
4aqv, 4aqw – hKIF11 (mutant) + tubulin + GDP + GTP + Mg – Cryo EM
4ck5, 4ck6, 4ck7 – hKIF11 (mutant) + tubulin + ADP + GDP + GTP + Mg – Cryo EM

Kinesin-14

1f9t, 1f9u, 1f9v, 1f9w, 3kar - Kar3 MD (mutant) - yeast
3l1c – DmNCD (mutant)

KCBP

3h4s – KCBP+ADP+Mg – Arabidopsis thaliana

NOD

3dc4 – DmNOD catalytic core domain+ADP
3dcb - DmNOD catalytic core domain+AMPPNP
3dco - DmNOD catalytic core domain+ cTubulin A+cTubulin B