1e93

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[[Image:1e93.gif|left|200px]]<br /><applet load="1e93" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1e93.gif|left|200px]]
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caption="1e93, resolution 2.00&Aring;" />
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'''HIGH RESOLUTION STRUCTURE AND BIOCHEMICAL PROPERTIES OF A RECOMBINANT CATALASE DEPLETED IN IRON'''<br />
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{{Structure
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|PDB= 1e93 |SIZE=350|CAPTION= <scene name='initialview01'>1e93</scene>, resolution 2.00&Aring;
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|SITE= <scene name='pdbsite=HEM:Hem+Binding+Site+For+Chain+A+HIS+A+54+Is+The+Distal+HIS+...'>HEM</scene>
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6]
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|GENE= KATA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=584 Proteus mirabilis])
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}}
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'''HIGH RESOLUTION STRUCTURE AND BIOCHEMICAL PROPERTIES OF A RECOMBINANT CATALASE DEPLETED IN IRON'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1E93 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_mirabilis Proteus mirabilis] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] Known structural/functional Site: <scene name='pdbsite=HEM:Hem+Binding+Site+For+Chain+A+HIS+A+54+Is+The+Distal+HIS+...'>HEM</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E93 OCA].
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1E93 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_mirabilis Proteus mirabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E93 OCA].
==Reference==
==Reference==
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Ferryl intermediates of catalase captured by time-resolved Weissenberg crystallography and UV-VIS spectroscopy., Gouet P, Jouve HM, Williams PA, Andersson I, Andreoletti P, Nussaume L, Hajdu J, Nat Struct Biol. 1996 Nov;3(11):951-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8901874 8901874]
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Ferryl intermediates of catalase captured by time-resolved Weissenberg crystallography and UV-VIS spectroscopy., Gouet P, Jouve HM, Williams PA, Andersson I, Andreoletti P, Nussaume L, Hajdu J, Nat Struct Biol. 1996 Nov;3(11):951-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8901874 8901874]
[[Category: Catalase]]
[[Category: Catalase]]
[[Category: Proteus mirabilis]]
[[Category: Proteus mirabilis]]
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[[Category: peroxidase]]
[[Category: peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:25:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:51:53 2008''

Revision as of 08:51, 20 March 2008

Image:1e93.gif


PDB ID 1e93

Drag the structure with the mouse to rotate
, resolution 2.00Å
Sites:
Ligands: , and
Gene: KATA (Proteus mirabilis)
Activity: Catalase, with EC number 1.11.1.6
Coordinates: save as pdb, mmCIF, xml



HIGH RESOLUTION STRUCTURE AND BIOCHEMICAL PROPERTIES OF A RECOMBINANT CATALASE DEPLETED IN IRON


Overview

Various enzymes use semi-stable ferryl intermediates and free radicals during their catalytic cycle, amongst them haem catalases. Structures for two transient intermediates (compounds I and II) of the NADPH-dependent catalase from Proteus mirabilis (PMC) have been determined by time-resolved X-ray crystallography and single crystal microspectrophotometry. The results show the formation and transformation of the ferryl group in the haem, and the unexpected binding of an anion during this reaction at a site distant from the haem.

About this Structure

1E93 is a Single protein structure of sequence from Proteus mirabilis. Full crystallographic information is available from OCA.

Reference

Ferryl intermediates of catalase captured by time-resolved Weissenberg crystallography and UV-VIS spectroscopy., Gouet P, Jouve HM, Williams PA, Andersson I, Andreoletti P, Nussaume L, Hajdu J, Nat Struct Biol. 1996 Nov;3(11):951-6. PMID:8901874

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