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1ear
From Proteopedia
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| - | [[Image:1ear.gif|left|200px]] | + | [[Image:1ear.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.7 A. TYPE II CRYSTAL FORM.''' | + | {{Structure |
| + | |PDB= 1ear |SIZE=350|CAPTION= <scene name='initialview01'>1ear</scene>, resolution 1.7Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A+Symmetry+Related+Subunits+Co+...'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.7 A. TYPE II CRYSTAL FORM.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EAR is a [ | + | 1EAR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EAR OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii., Remaut H, Safarov N, Ciurli S, Van Beeumen J, J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:[http:// | + | Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii., Remaut H, Safarov N, Ciurli S, Van Beeumen J, J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11602602 11602602] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sporosarcina pasteurii]] | [[Category: Sporosarcina pasteurii]] | ||
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[[Category: urease operon]] | [[Category: urease operon]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:52:46 2008'' |
Revision as of 08:52, 20 March 2008
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| , resolution 1.7Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.7 A. TYPE II CRYSTAL FORM.
Overview
Bacillus pasteurii UreE (BpUreE) is a putative chaperone assisting the insertion of Ni(2+) ions in the active site of urease. The x-ray structure of the protein has been determined for two crystal forms, at 1.7 and 1.85 A resolution, using SIRAS phases derived from a Hg(2+)-derivative. BpUreE is composed of distinct N- and C-terminal domains, connected by a short flexible linker. The structure reveals the topology of an elongated homodimer, formed by interaction of the two C-terminal domains through hydrophobic interactions. A single Zn(2+) ion bound to four conserved His-100 residues, one from each monomer, connects two dimers resulting in a tetrameric BpUreE known to be formed in concentrated solutions. The Zn(2+) ion can be replaced by Ni(2+) as shown by anomalous difference maps obtained on a crystal of BpUreE soaked in a solution containing NiCl(2). A large hydrophobic patch surrounding the metal ion site is surface-exposed in the biologically relevant dimer. The BpUreE structure represents the first for this class of proteins and suggests a possible role for UreE in the urease nickel-center assembly.
About this Structure
1EAR is a Single protein structure of sequence from Sporosarcina pasteurii. Full crystallographic information is available from OCA.
Reference
Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii., Remaut H, Safarov N, Ciurli S, Van Beeumen J, J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:11602602
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