4hic
From Proteopedia
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| - | + | ==Crystal structure of the potential transfer protein TraK from Gram-positive conjugative plasmid pIP501== | |
| - | + | <StructureSection load='4hic' size='340' side='right' caption='[[4hic]], [[Resolution|resolution]] 3.00Å' scene=''> | |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4hic]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HIC FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF11 from conjugative plasmid pIP501 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hic RCSB], [http://www.ebi.ac.uk/pdbsum/4hic PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Conjugative plasmid transfer presents a serious threat to human health as the most important means of spreading antibiotic resistance and virulence genes among bacteria. The required direct cell-cell contact is established by a multi-protein complex, the conjugative type IV secretion system (T4SS). The conjugative core complex spans the cellular envelope and serves as a channel for macromolecular secretion. T4SSs of Gram-negative (G-) origin have been studied in great detail. In contrast, T4SSs of Gram-positive (G+) bacteria have only received little attention thus far, despite the medical relevance of numerous G+ pathogens (e.g. enterococci, staphylococci and streptococci). This study provides structural information on the type IV secretion (T4S) protein TraK of the G+ broad host range Enterococcus conjugative plasmid pIP501. The crystal structure of the N-terminally truncated construct TraKDelta was determined to 3.0 A resolution and exhibits a novel fold. Immunolocalization demonstrated that the protein localizes to the cell wall facing towards the cell exterior, but does not exhibit surface accessibility. Circular dichroism, dynamic light scattering and size-exclusion chromatography confirmed the protein to be a monomer. With the exception of proteins from closely related T4SSs, no significant sequence or structural relatives were found. This observation marks the protein as a very exclusive, specialized member of the pIP501 T4SS. | ||
| - | + | The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold.,Goessweiner-Mohr N, Fercher C, Arends K, Birner-Gruenberger R, Laverde-Gomez D, Huebner J, Grohmann E, Keller W Acta Crystallogr D Biol Crystallogr. 2014 Apr;70(Pt 4):1124-35. doi:, 10.1107/S1399004714001606. Epub 2014 Mar 21. PMID:24699656<ref>PMID:24699656</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]] | ||
[[Category: Goessweiner-Mohr, N.]] | [[Category: Goessweiner-Mohr, N.]] | ||
[[Category: Keller, W.]] | [[Category: Keller, W.]] | ||
Revision as of 05:31, 24 September 2014
Crystal structure of the potential transfer protein TraK from Gram-positive conjugative plasmid pIP501
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