3wjm
From Proteopedia
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| - | ''' | + | ==Crystal structure of Bombyx mori Sp2/Sp3 heterohexamer== |
| + | <StructureSection load='3wjm' size='340' side='right' caption='[[3wjm]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3wjm]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WJM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WJM FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wjm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wjm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wjm RCSB], [http://www.ebi.ac.uk/pdbsum/3wjm PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In holometabolous insects, the accumulation and utilization of storage proteins (SPs), including arylphorins and methionine-rich proteins, are critical for the insect metamorphosis. SPs function as amino acids reserves, which are synthesized in fat body, secreted into the larval hemolymph and taken up by fat body shortly before pupation. However, the detailed molecular mechanisms of digestion and utilization of SPs during development are largely unknown. Here, we report the crystal structure of Bombyx mori arylphorins at 2.8 A, which displays a heterohexameric structural arrangement formed by trimerization of dimers comprising two structural similar arylphorins. Our limited proteolysis assay and microarray data strongly suggest that papain-like proteases are the major players for B. mori arylphorins digestion in vitro and in vivo. Consistent with the biochemical data, dozens of papain cleavage sites are mapped on the surface of the heterohexameric structure of B. mori arylphorins. Hence, our results provide the insightful information to understand the metamorphosis of holometabolous insects at molecular level. | ||
| - | + | Crystal structure of Bombyx mori arylphorins reveals a 3:3 heterohexamer with multiple papain cleavage sites.,Hou Y, Li J, Li Y, Dong Z, Xia Q, Yuan YA Protein Sci. 2014 Jun;23(6):735-46. doi: 10.1002/pro.2457. Epub 2014 Apr 2. PMID:24639361<ref>PMID:24639361</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bombyx mori]] | ||
| + | [[Category: Hou, Y.]] | ||
| + | [[Category: Yuan, Y A.]] | ||
| + | [[Category: Bombyx mori storage protein]] | ||
| + | [[Category: Heterohexamer]] | ||
| + | [[Category: Oxygen transport]] | ||
| + | [[Category: Papain cleavage site]] | ||
Revision as of 10:36, 24 September 2014
Crystal structure of Bombyx mori Sp2/Sp3 heterohexamer
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