3wla
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal Structure of sOPH Native== |
| + | <StructureSection load='3wla' size='340' side='right' caption='[[3wla]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3wla]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WLA FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wl5|3wl5]], [[3wl6|3wl6]], [[3wl7|3wl7]], [[3wl8|3wl8]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-diketone_hydrolase Beta-diketone hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.7 3.7.1.7] </span></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wla OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wla RCSB], [http://www.ebi.ac.uk/pdbsum/3wla PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The ever-increasing production and use of polyvinyl alcohol (PVA) threaten our environment. Yet PVA can be assimilated by microbes in two steps: oxidation and cleavage. Here we report novel alpha/beta-hydrolase structures of oxidized PVA hydrolase (OPH) from two known PVA-degrading organisms, Sphingopyxis sp. 113P3 and Pseudomonas sp. VM15C, including complexes with substrate analogues, acetylacetone and caprylate. The active site is covered by a lid-like beta-ribbon. Unlike other esterase and amidase, OPH is unique in cleaving the CC bond of beta-diketone, although it has a catalytic triad similar to that of most alpha/beta-hydrolases. Analysis of the crystal structures suggests a double-oxyanion-hole mechanism, previously only found in thiolase cleaving beta-ketoacyl-CoA. Three mutations in the lid region showed enhanced activity, with potential in industrial applications. | ||
| - | + | Structural insights into enzymatic degradation of oxidized polyvinyl alcohol.,Yang Y, Ko TP, Liu L, Li J, Huang CH, Chan HC, Ren F, Jia D, Wang AH, Guo RT, Chen J, Du G Chembiochem. 2014 Sep 5;15(13):1882-6. doi: 10.1002/cbic.201402166. Epub 2014 Jul, 8. PMID:25044912<ref>PMID:25044912</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Beta-diketone hydrolase]] | ||
| + | [[Category: Chan, H C.]] | ||
| + | [[Category: Chen, J.]] | ||
| + | [[Category: Du, G C.]] | ||
| + | [[Category: Guo, R T.]] | ||
| + | [[Category: Huang, C H.]] | ||
| + | [[Category: Jia, D X.]] | ||
| + | [[Category: Ko, T P.]] | ||
| + | [[Category: Li, J H.]] | ||
| + | [[Category: Liu, L.]] | ||
| + | [[Category: Ren, F F.]] | ||
| + | [[Category: Wang, A H.J.]] | ||
| + | [[Category: Yang, Y.]] | ||
| + | [[Category: Alpha/beta-hydrolase]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Oxi-polyvinyl alcohol hydrolase]] | ||
Revision as of 10:36, 24 September 2014
Crystal Structure of sOPH Native
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