1ekb
From Proteopedia
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| - | [[Image:1ekb.jpg|left|200px]] | + | [[Image:1ekb.jpg|left|200px]] |
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| - | '''THE SERINE PROTEASE DOMAIN OF ENTEROPEPTIDASE BOUND TO INHIBITOR VAL-ASP-ASP-ASP-ASP-LYS-CHLOROMETHANE''' | + | {{Structure |
| + | |PDB= 1ekb |SIZE=350|CAPTION= <scene name='initialview01'>1ekb</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CH2:METHYLENE GROUP'>CH2</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Enteropeptidase Enteropeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.9 3.4.21.9] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE SERINE PROTEASE DOMAIN OF ENTEROPEPTIDASE BOUND TO INHIBITOR VAL-ASP-ASP-ASP-ASP-LYS-CHLOROMETHANE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EKB is a [ | + | 1EKB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EKB OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide., Lu D, Futterer K, Korolev S, Zheng X, Tan K, Waksman G, Sadler JE, J Mol Biol. 1999 Sep 17;292(2):361-73. PMID:[http:// | + | Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide., Lu D, Futterer K, Korolev S, Zheng X, Tan K, Waksman G, Sadler JE, J Mol Biol. 1999 Sep 17;292(2):361-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10493881 10493881] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Enteropeptidase]] | [[Category: Enteropeptidase]] | ||
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[[Category: trypsinogen activation]] | [[Category: trypsinogen activation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:56:45 2008'' |
Revision as of 08:56, 20 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Enteropeptidase, with EC number 3.4.21.9 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE SERINE PROTEASE DOMAIN OF ENTEROPEPTIDASE BOUND TO INHIBITOR VAL-ASP-ASP-ASP-ASP-LYS-CHLOROMETHANE
Overview
Enteropeptidase is a membrane-bound serine protease that initiates the activation of pancreatic hydrolases by cleaving and activating trypsinogen. The enzyme is remarkably specific and cleaves after lysine residues of peptidyl substrates that resemble trypsinogen activation peptides such as Val-(Asp)4-Lys. To characterize the determinants of substrate specificity, we solved the crystal structure of the bovine enteropeptidase catalytic domain to 2.3 A resolution in complex with the inhibitor Val-(Asp)4-Lys-chloromethane. The catalytic mechanism and contacts with lysine at substrate position P1 are conserved with other trypsin-like serine proteases. However, the aspartyl residues at positions P2-P4 of the inhibitor interact with the enzyme surface mainly through salt bridges with the Nzeta atom of Lys99. Mutation of Lys99 to Ala, or acetylation with acetic anhydride, specifically prevented the cleavage of trypsinogen or Gly-(Asp)4-Lys-beta-naphthylamide and reduced the rate of inhibition by Val-(Asp)4-Lys-chloromethane 22 to 90-fold. For these reactions, Lys99 was calculated to account for 1.8 to 2.5 kcal mol(-1) of the free energy of transition state binding. Thus, a unique basic exosite on the enteropeptidase surface has evolved to facilitate the cleavage of its physiological substrate, trypsinogen.
About this Structure
1EKB is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.
Reference
Crystal structure of enteropeptidase light chain complexed with an analog of the trypsinogen activation peptide., Lu D, Futterer K, Korolev S, Zheng X, Tan K, Waksman G, Sadler JE, J Mol Biol. 1999 Sep 17;292(2):361-73. PMID:10493881
Page seeded by OCA on Thu Mar 20 10:56:45 2008
