1elt
From Proteopedia
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| - | [[Image:1elt.gif|left|200px]] | + | [[Image:1elt.gif|left|200px]] |
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| - | '''STRUCTURE OF NATIVE PANCREATIC ELASTASE FROM NORTH ATLANTIC SALMON AT 1.61 ANGSTROMS RESOLUTION''' | + | {{Structure |
| + | |PDB= 1elt |SIZE=350|CAPTION= <scene name='initialview01'>1elt</scene>, resolution 1.61Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF NATIVE PANCREATIC ELASTASE FROM NORTH ATLANTIC SALMON AT 1.61 ANGSTROMS RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ELT is a [ | + | 1ELT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELT OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of native pancreatic elastase from North Atlantic salmon at 1.61 A resolution., Berglund GI, Willassen NP, Hordvik A, Smalas AO, Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):925-37. PMID:[http:// | + | Structure of native pancreatic elastase from North Atlantic salmon at 1.61 A resolution., Berglund GI, Willassen NP, Hordvik A, Smalas AO, Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):925-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299762 15299762] |
[[Category: Pancreatic elastase]] | [[Category: Pancreatic elastase]] | ||
[[Category: Salmo salar]] | [[Category: Salmo salar]] | ||
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[[Category: serine proteinase]] | [[Category: serine proteinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:57:19 2008'' |
Revision as of 08:57, 20 March 2008
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| , resolution 1.61Å | |||||||
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| Ligands: | |||||||
| Activity: | Pancreatic elastase, with EC number 3.4.21.36 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF NATIVE PANCREATIC ELASTASE FROM NORTH ATLANTIC SALMON AT 1.61 ANGSTROMS RESOLUTION
Overview
The crystal structure of native salmon pancreatic elastase (SPE) has been solved by molecular-replacement methods, and refined by conventional conjugate-gradient methods and simulated-annealing techniques. The final R value is 17.2% for 21 389 reflections between 8.0 and 1.61 A, and the corresponding free R value is 23.9%. The overall tertiary structure of SPE is remarkably similar to that of porcine pancreatic elastase I (PPE), to which it shows about 67% sequence identity. The primary structure of SPE is determined from the electron-density maps, and only about 15 side chains are somewhat uncertain. Interesting differences between SPE and PPE, are one sequence deletion assigned to position 186, the residue 192 at the entrance of the specificity pocket is substituted from a Gln in PPE to Asn in SPE, and one of the calcium ligands is different. Furthermore, electron density is missing in SPE for the last three residues of the C-terminal helix. A comparison of the present amino-acid sequence of SPE with other sequences available indicates that SPE belongs to the class 1 pancreatic elastases.
About this Structure
1ELT is a Single protein structure of sequence from Salmo salar. Full crystallographic information is available from OCA.
Reference
Structure of native pancreatic elastase from North Atlantic salmon at 1.61 A resolution., Berglund GI, Willassen NP, Hordvik A, Smalas AO, Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):925-37. PMID:15299762
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