1ht2

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[[Image:1ht2.png|left|200px]]
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==Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU==
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<StructureSection load='1ht2' size='340' side='right' caption='[[1ht2]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ht2]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21(de3) Escherichia coli bl21(de3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1HT2 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1e94|1e94]], [[1doo|1doo]], [[1hqy|1hqy]], [[1ht1|1ht1]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ht2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ht2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ht2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ht2 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/1ht2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: The bacterial heat shock locus ATPase HslU is an AAA(+) protein that has structures known in many nucleotide-free and -bound states. Nucleotide is required for the formation of the biologically active HslU hexameric assembly. The hexameric HslU ATPase binds the dodecameric HslV peptidase and forms an ATP-dependent HslVU protease. RESULTS: We have characterized four distinct HslU conformational states, going sequentially from open to closed: the empty, SO(4), ATP, and ADP states. The nucleotide binds at a cleft formed by an alpha/beta domain and an alpha-helical domain in HslU. The four HslU states differ by a rotation of the alpha-helical domain. This classification leads to a correction of nucleotide identity in one structure and reveals the ATP hydrolysis-dependent structural changes in the HslVU complex, including a ring rotation and a conformational change of the HslU C terminus. This leads to an amended protein unfolding-coupled translocation mechanism. CONCLUSIONS: The observed nucleotide-dependent conformational changes in HslU and their governing principles provide a framework for the mechanistic understanding of other AAA(+) proteins.
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{{STRUCTURE_1ht2| PDB=1ht2 | SCENE= }}
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Nucleotide-dependent conformational changes in a protease-associated ATPase HsIU.,Wang J, Song JJ, Seong IS, Franklin MC, Kamtekar S, Eom SH, Chung CH Structure. 2001 Nov;9(11):1107-16. PMID:11709174<ref>PMID:11709174</ref>
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===Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_11709174}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1ht2]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21(de3) Escherichia coli bl21(de3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT2 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:011709174</ref><references group="xtra"/>
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[[Category: Chung, C H.]]
[[Category: Chung, C H.]]
[[Category: Eom, S H.]]
[[Category: Eom, S H.]]

Revision as of 08:38, 28 September 2014

Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU

1ht2, resolution 2.80Å

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