1ka8

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[[Image:1ka8.png|left|200px]]
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==Crystal Structure of the Phage P4 Origin-Binding Domain==
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<StructureSection load='1ka8' size='340' side='right' caption='[[1ka8]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ka8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p4 Enterobacteria phage p4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KA8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1KA8 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ka8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ka8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ka8 RCSB], [http://www.ebi.ac.uk/pdbsum/1ka8 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ka/1ka8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The origin-binding domain of the gpalpha protein of phage P4 (P4-OBD) mediates origin recognition and regulation of gpalpha activity by the protein Cnr. We have determined the crystal structure of P4-OBD at 2.95 A resolution. The structure of P4-OBD is that of a dimer with pseudo twofold symmetry. Each subunit has a winged helix topology with a unique structure among initiator proteins. The only structural homologue of the P4-OBD subunit is the DNA-binding domain of the eukaryotic transcriptional activator Rfx1. Based on this structural alignment, a model for origin recognition by the P4-OBD dimer is suggested. P4-OBD mutations that interfere with Cnr binding locate to the dimer interface, indicating that Cnr acts by disrupting the gpalpha dimer. P4-OBD dimerization is mediated by helices alpha1 and alpha3 in both subunits, a mode of winged helix protein dimerization that is reminiscent of that of the eukaryotic transcription factors E2F and DP. This, in turn, suggests that Cnr is also a winged helix protein, a possibility that is supported by previously unreported sequence homologies between Cnr and Rfx1 and homology modelling. Hence, in a mechanism that appears to be conserved from phage to man, the DNA-binding activity of winged helix proteins can be regulated by other winged helix proteins via the versatile use of the winged helix motif as a homo- or heterodimerization scaffold.
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{{STRUCTURE_1ka8| PDB=1ka8 | SCENE= }}
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Phage P4 origin-binding domain structure reveals a mechanism for regulation of DNA-binding activity by homo- and heterodimerization of winged helix proteins.,Yeo HJ, Ziegelin G, Korolev S, Calendar R, Lanka E, Waksman G Mol Microbiol. 2002 Feb;43(4):855-67. PMID:11929537<ref>PMID:11929537</ref>
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===Crystal Structure of the Phage P4 Origin-Binding Domain===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_11929537}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1ka8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p4 Enterobacteria phage p4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KA8 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:011929537</ref><references group="xtra"/>
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[[Category: Enterobacteria phage p4]]
[[Category: Enterobacteria phage p4]]
[[Category: Calendar, R.]]
[[Category: Calendar, R.]]

Revision as of 09:00, 28 September 2014

Crystal Structure of the Phage P4 Origin-Binding Domain

1ka8, resolution 2.95Å

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