1eqx
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1eqx.jpg|left|200px]] | + | [[Image:1eqx.jpg|left|200px]] |
| - | + | ||
| - | '''SOLUTION STRUCTURE DETERMINATION AND MUTATIONAL ANALYSIS OF THE PAPILLOMAVIRUS E6-INTERACTING PEPTIDE OF E6AP''' | + | {{Structure |
| + | |PDB= 1eqx |SIZE=350|CAPTION= <scene name='initialview01'>1eqx</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''SOLUTION STRUCTURE DETERMINATION AND MUTATIONAL ANALYSIS OF THE PAPILLOMAVIRUS E6-INTERACTING PEPTIDE OF E6AP''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1EQX is a [ | + | 1EQX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQX OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure determination and mutational analysis of the papillomavirus E6 interacting peptide of E6AP., Be X, Hong Y, Wei J, Androphy EJ, Chen JJ, Baleja JD, Biochemistry. 2001 Feb 6;40(5):1293-9. PMID:[http:// | + | Solution structure determination and mutational analysis of the papillomavirus E6 interacting peptide of E6AP., Be X, Hong Y, Wei J, Androphy EJ, Chen JJ, Baleja JD, Biochemistry. 2001 Feb 6;40(5):1293-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11170455 11170455] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: peptide]] | [[Category: peptide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:59:11 2008'' |
Revision as of 08:59, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE DETERMINATION AND MUTATIONAL ANALYSIS OF THE PAPILLOMAVIRUS E6-INTERACTING PEPTIDE OF E6AP
Contents |
Overview
E6AP is a cellular protein that binds cancer-related papillomaviral E6 proteins. The E6 binding domain, called E6ap, is located on an 18-amino acid segment of E6AP. The corresponding peptide was synthesized and its structure determined by nuclear magnetic resonance spectroscopy. The overall structure of the peptide is helical. A consensus E6-binding sequence among different E6 interacting proteins contains three conserved hydrophobic residues. In the structure of the E6AP peptide, the three conserved leucines (Leu 9, Leu 12, and Leu 13) form a hydrophobic patch on one face of the alpha-helix. Substitution of any of these leucines with alanine abolished binding to E6 protein, indicating that the entire hydrophobic patch is necessary. Mutation of a glutamate to proline, but not alanine, also disrupted the interaction between E6 and E6AP protein, suggesting that the E6-binding motif of the E6AP protein must be helical when bound to E6. Comparison of the E6ap structure and mutational results with those of another E6-binding protein (E6BP/ERC-55) indicates the existence of a general E6-binding motif.
Disease
Known disease associated with this structure: Angelman syndrome OMIM:[601623]
About this Structure
1EQX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure determination and mutational analysis of the papillomavirus E6 interacting peptide of E6AP., Be X, Hong Y, Wei J, Androphy EJ, Chen JJ, Baleja JD, Biochemistry. 2001 Feb 6;40(5):1293-9. PMID:11170455
Page seeded by OCA on Thu Mar 20 10:59:11 2008
Categories: Homo sapiens | Single protein | Androphy, E J. | Baleja, J D. | Be, X. | Chen, J J. | Hong, Y. | Alpha helix | Peptide
