1erj
From Proteopedia
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| - | [[Image:1erj.jpg|left|200px]] | + | [[Image:1erj.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1''' | + | {{Structure |
| + | |PDB= 1erj |SIZE=350|CAPTION= <scene name='initialview01'>1erj</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ERJ is a [ | + | 1ERJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERJ OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast., Sprague ER, Redd MJ, Johnson AD, Wolberger C, EMBO J. 2000 Jun 15;19(12):3016-27. PMID:[http:// | + | Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast., Sprague ER, Redd MJ, Johnson AD, Wolberger C, EMBO J. 2000 Jun 15;19(12):3016-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10856245 10856245] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: beta-propeller]] | [[Category: beta-propeller]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:59:22 2008'' |
Revision as of 08:59, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1
Overview
The Tup1-Ssn6 corepressor complex regulates the expression of several sets of genes, including genes that specify mating type in the yeast Saccharomyces cerevisiae. Repression of mating-type genes occurs when Tup1-Ssn6 is brought to the DNA by the Matalpha2 DNA-binding protein and assembled upstream of a- and haploid-specific genes. We have determined the 2.3 A X-ray crystal structure of the C-terminal domain of Tup1 (accesion No. 1ERJ), a 43 kDa fragment that contains seven copies of the WD40 sequence motif and binds to the Matalpha2 protein. Moreover, this portion of the protein can partially substitute for full-length Tup1 in bringing about transcriptional repression. The structure reveals a seven-bladed beta propeller with an N-terminal subdomain that is anchored to the side of the propeller and extends the beta sheet of one of the blades. Point mutations in Tup1 that specifically affect the Tup1-Matalpha2 interaction cluster on one surface of the propeller. We identified regions of Tup1 that are conserved among the fungal Tup1 homologs and may be important in protein-protein interactions with additional components of the Tup1-mediated repression pathways.
About this Structure
1ERJ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast., Sprague ER, Redd MJ, Johnson AD, Wolberger C, EMBO J. 2000 Jun 15;19(12):3016-27. PMID:10856245
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