1etj
From Proteopedia
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| - | [[Image:1etj.gif|left|200px]] | + | [[Image:1etj.gif|left|200px]] |
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| - | '''AZURIN MUTANT WITH MET 121 REPLACED BY GLU''' | + | {{Structure |
| + | |PDB= 1etj |SIZE=350|CAPTION= <scene name='initialview01'>1etj</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''AZURIN MUTANT WITH MET 121 REPLACED BY GLU''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ETJ is a [ | + | 1ETJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ETJ OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray structure determination and characterization of the Pseudomonas aeruginosa azurin mutant Met121Glu., Karlsson BG, Tsai LC, Nar H, Sanders-Loehr J, Bonander N, Langer V, Sjolin L, Biochemistry. 1997 Apr 8;36(14):4089-95. PMID:[http:// | + | X-ray structure determination and characterization of the Pseudomonas aeruginosa azurin mutant Met121Glu., Karlsson BG, Tsai LC, Nar H, Sanders-Loehr J, Bonander N, Langer V, Sjolin L, Biochemistry. 1997 Apr 8;36(14):4089-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9100002 9100002] |
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: periplasmic]] | [[Category: periplasmic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:00:04 2008'' |
Revision as of 09:00, 20 March 2008
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| , resolution 2.3Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
AZURIN MUTANT WITH MET 121 REPLACED BY GLU
Overview
The Met121Glu azurin mutant has been crystallized and the structure determined at a resolution of 2.3 A. In the crystal structure a carboxyl oxygen of Met121Glu is coordinated to the metal at a distance of 2.2 A. Single-crystal resonance Raman spectroscopy was used to show that the glutamic acid residue in the copper site was in the protonated state. Titration of this residue gives rise to a number of unusual, pH-dependent properties: as the pH is increased from 4 to 8, the S(Cys)-Cu ligand-to-metal charge transfer bands are blue shifted and their intensity ratio is reversed, the EPR signal changes from type 1 copper to a new form of protein-bound copper, and the redox potential changes from 370 to 180 mV. The spectroscopic changes in this pH interval are consistent with a two-state model. From the pH dependence of the optical and EPR spectra, pKa = 5.0 for the glutamic acid in the oxidized protein was determined.
About this Structure
1ETJ is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
X-ray structure determination and characterization of the Pseudomonas aeruginosa azurin mutant Met121Glu., Karlsson BG, Tsai LC, Nar H, Sanders-Loehr J, Bonander N, Langer V, Sjolin L, Biochemistry. 1997 Apr 8;36(14):4089-95. PMID:9100002
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