1evi

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[[Image:1evi.gif|left|200px]]<br /><applet load="1evi" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1evi.gif|left|200px]]
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caption="1evi, resolution 2.5&Aring;" />
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'''THREE-DIMENSIONAL STRUCTURE OF THE PURPLE INTERMEDIATE OF PORCINE KIDNEY D-AMINO ACID OXIDASE'''<br />
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{{Structure
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|PDB= 1evi |SIZE=350|CAPTION= <scene name='initialview01'>1evi</scene>, resolution 2.5&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=2PC:3,4-DIHYDRO-2H-PYRROLIUM-5-CARBOXYLATE'>2PC</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/D-amino-acid_oxidase D-amino-acid oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.3 1.4.3.3]
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|GENE=
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}}
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'''THREE-DIMENSIONAL STRUCTURE OF THE PURPLE INTERMEDIATE OF PORCINE KIDNEY D-AMINO ACID OXIDASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1EVI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=2PC:'>2PC</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/D-amino-acid_oxidase D-amino-acid oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.3 1.4.3.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVI OCA].
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1EVI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVI OCA].
==Reference==
==Reference==
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Three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase. Optimization of the oxidative half-reaction through alignment of the product with reduced flavin., Mizutani H, Miyahara I, Hirotsu K, Nishina Y, Shiga K, Setoyama C, Miura R, J Biochem. 2000 Jul;128(1):73-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10876160 10876160]
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Three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase. Optimization of the oxidative half-reaction through alignment of the product with reduced flavin., Mizutani H, Miyahara I, Hirotsu K, Nishina Y, Shiga K, Setoyama C, Miura R, J Biochem. 2000 Jul;128(1):73-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10876160 10876160]
[[Category: D-amino-acid oxidase]]
[[Category: D-amino-acid oxidase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: flavoenzyme]]
[[Category: flavoenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:31:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:00:50 2008''

Revision as of 09:00, 20 March 2008

Image:1evi.gif


PDB ID 1evi

Drag the structure with the mouse to rotate
, resolution 2.5Å
Ligands: and
Activity: D-amino-acid oxidase, with EC number 1.4.3.3
Coordinates: save as pdb, mmCIF, xml



THREE-DIMENSIONAL STRUCTURE OF THE PURPLE INTERMEDIATE OF PORCINE KIDNEY D-AMINO ACID OXIDASE


Overview

The three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase (DAO) was solved by cryo-X-ray crystallography; the purple intermediate is known to comprise a complex between the dehydrogenated product, an imino acid, and the reduced form of DAO. The crystalline purple intermediate was obtained by anaerobically soaking crystals of oxidized DAO in a buffer containing excess D-proline as the substrate. The dehydrogenated product, delta(1)-pyrrolidine-2-carboxylate (DPC), is found sandwiched between the phenol ring of Tyr 224 and the planar reduced flavin ring. The cationic protonated imino nitrogen is within hydrogen-bonding distance of the backbone carbonyl oxygen of Gly 313. The carboxyl group of DPC is recognized by the Arg 283 guanidino and Tyr 228 hydroxyl groups through ion-pairing and hydrogen-bonding, respectively. The (+)HN=C double bond of DPC overlaps the N(5)-C(4a) bond of reduced flavin. The electrostatic effect of the cationic nitrogen of DPC is suggested to shift the resonance hybridization of anionic reduced flavin toward a canonical form with a negative charge at C(4a), thereby augmenting the electron density at C(4a), from which electrons are transferred to molecular oxygen during reoxidation of reduced flavin. The reactivity of reduced flavin in the purple intermediate, therefore, is enhanced through the alignment of DPC with respect to reduced flavin.

About this Structure

1EVI is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase. Optimization of the oxidative half-reaction through alignment of the product with reduced flavin., Mizutani H, Miyahara I, Hirotsu K, Nishina Y, Shiga K, Setoyama C, Miura R, J Biochem. 2000 Jul;128(1):73-81. PMID:10876160

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