1eyn
From Proteopedia
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| - | [[Image:1eyn.jpg|left|200px]] | + | [[Image:1eyn.jpg|left|200px]] |
| - | + | ||
| - | '''STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS''' | + | {{Structure |
| + | |PDB= 1eyn |SIZE=350|CAPTION= <scene name='initialview01'>1eyn</scene>, resolution 1.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=2AN:1-ANILINO-8-NAPHTHALENE+SULFONATE'>2AN</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/UDP-N-acetylglucosamine_1-carboxyvinyltransferase UDP-N-acetylglucosamine 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.7 2.5.1.7] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EYN is a [ | + | 1EYN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYN OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA., Schonbrunn E, Eschenburg S, Luger K, Kabsch W, Amrhein N, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6345-9. PMID:[http:// | + | Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA., Schonbrunn E, Eschenburg S, Luger K, Kabsch W, Amrhein N, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6345-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10823915 10823915] |
[[Category: Enterobacter cloacae]] | [[Category: Enterobacter cloacae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: inside-out alpha-beta barrel; l-isoaspartate in position 67]] | [[Category: inside-out alpha-beta barrel; l-isoaspartate in position 67]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:02:00 2008'' |
Revision as of 09:02, 20 March 2008
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| , resolution 1.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | UDP-N-acetylglucosamine 1-carboxyvinyltransferase, with EC number 2.5.1.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF MURA LIGANDED WITH THE EXTRINSIC FLUORESCENCE PROBE ANS
Overview
The extrinsic fluorescence dye 8-anilino-1-naphthalene sulfonate (ANS) is widely used for probing conformational changes in proteins, yet no detailed structure of ANS bound to any protein has been reported so far. ANS has been successfully used to monitor the induced-fit mechanism of MurA [UDPGlcNAc enolpyruvyltransferase (EC )], an essential enzyme for bacterial cell wall biosynthesis. We have solved the crystal structure of the ANS small middle dotMurA complex at 1.7-A resolution. ANS binds at an originally solvent-exposed region near Pro-112 and induces a major restructuring of the loop Pro-112-Pro-121, such that a specific binding site emerges. The fluorescence probe is sandwiched between the strictly conserved residues Arg-91, Pro-112, and Gly-113. Substrate binding to MurA is accompanied by large movements especially of the loop and Arg-91, which explains why ANS is an excellent sensor of conformational changes during catalysis of this pharmaceutically important enzyme.
About this Structure
1EYN is a Single protein structure of sequence from Enterobacter cloacae. Full crystallographic information is available from OCA.
Reference
Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA., Schonbrunn E, Eschenburg S, Luger K, Kabsch W, Amrhein N, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6345-9. PMID:10823915
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