1eyu
From Proteopedia
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| - | [[Image:1eyu.gif|left|200px]] | + | [[Image:1eyu.gif|left|200px]] |
| - | + | ||
| - | '''HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6''' | + | {{Structure |
| + | |PDB= 1eyu |SIZE=350|CAPTION= <scene name='initialview01'>1eyu</scene>, resolution 1.78Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EYU is a [ | + | 1EYU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYU OCA]. |
==Reference== | ==Reference== | ||
| - | PvuII endonuclease contains two calcium ions in active sites., Horton JR, Cheng X, J Mol Biol. 2000 Jul 28;300(5):1049-56. PMID:[http:// | + | PvuII endonuclease contains two calcium ions in active sites., Horton JR, Cheng X, J Mol Biol. 2000 Jul 28;300(5):1049-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10903853 10903853] |
[[Category: Proteus vulgaris]] | [[Category: Proteus vulgaris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: restriction enzyme]] | [[Category: restriction enzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:02:05 2008'' |
Revision as of 09:02, 20 March 2008
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| , resolution 1.78Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIGH RESOLUTION STRUCTURE OF THE PVUII ENDONCULEASE/COGNATE DNA COMPLEX AT PH 4.6
Overview
Restriction endonucleases differ in their use of metal cofactors despite having remarkably similar folds for their catalytic regions. To explore this, we have characterized the interaction of endonuclease PvuII with the catalytically incompetent cation Ca(2+). The structure of a glutaraldehyde-crosslinked crystal of the endonuclease PvuII-DNA complex, determined in the presence of Ca(2+) at a pH of approximately 6.5, supports a two-metal mechanism of DNA cleavage by PvuII. The first Ca(2+) position matches that found in all structurally examined endonucleases, while the second position is similar to that of EcoRV but is distinct from that of BamHI and BglI. The location of the second metal in PvuII, unlike that in BamHI/BglI, permits no direct interaction between the second metal and the O3' oxygen leaving group. However, the interactions between the DNA scissile phosphate and the metals, the first metal and the attacking water, and the attacking water and DNA are the same in PvuII as they are in the two-metal models of BamHI and BglI, but are distinct from the proposed three-metal or the two-metal models of EcoRV.
About this Structure
1EYU is a Single protein structure of sequence from Proteus vulgaris. Full crystallographic information is available from OCA.
Reference
PvuII endonuclease contains two calcium ions in active sites., Horton JR, Cheng X, J Mol Biol. 2000 Jul 28;300(5):1049-56. PMID:10903853
Page seeded by OCA on Thu Mar 20 11:02:05 2008
