2iwf

From Proteopedia

Revision as of 15:12, 30 October 2007

RESTING FORM OF PINK NITROUS OXIDE REDUCTASE FROM ACHROMOBACTER CYCLOCLASTES

Overview

The difficult chemistry of nitrous oxide (N2O) reduction to gaseous, nitrogen (N2) in biology is catalysed by the novel micro4-sulphide-bridged, tetranuclear Cuz cluster of the N2O reductases (N2OR). Two, spectroscopically distinct forms of this cluster have been identified as, CuZ and CuZ*. We have obtained a 1.86 A resolution crystal structure of, the pink-purple species of N2OR from Achromobacter cycloclastes (AcN2OR), isolated under aerobic conditions. This structure reveals a previously, unobserved ligation with two oxygen atoms from H2O/OH- coordinated to Cu1, and Cu4 of the catalytic centre. We ascribe this structure to be that of, the CuZ form of the cluster, since the previously reported structures of, two blue species of N2ORs, also isolated aerobically, have characterised, the ... [(full description)]

About this Structure

2IWF is a [Single protein] structure of sequence from [Achromobacter cycloclastes] with CU, CA, CL, NA and S as [ligands]. Active as [Nitrous-oxide reductase], with EC number [1.7.99.6]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from Achromobacter cycloclastes., Paraskevopoulos K, Antonyuk SV, Sawers RG, Eady RR, Hasnain SS, J Mol Biol. 2006 Sep 8;362(1):55-65. Epub 2006 Jul 12. PMID:16904686

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