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1g3a
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| - | [[ | + | ==STRUCTURE OF RNA DUPLEXES (CIGCGICG)2== |
| + | <StructureSection load='1g3a' size='340' side='right' caption='[[1g3a]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1g3a]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G3A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1G3A FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IC:ISOCYTIDINE-5-MONOPHOSPHATE'>IC</scene>, <scene name='pdbligand=IG:ISOGUANOSINE-5-MONOPHOSPHATE'>IG</scene></td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g3a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g3a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1g3a RCSB], [http://www.ebi.ac.uk/pdbsum/1g3a PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Isoguanosine (iG) and isocytidine (iC) differ from guanosine (G) and cytidine (C), respectively, in that the amino and carbonyl groups are transposed. The thermodynamic properties of a set of iG, iC containing RNA duplexes have been measured by UV optical melting. It is found that iG-iC replacements usually stabilize duplexes, and the stabilization per iG-iC pair is sequence-dependent. The sequence dependence can be fit to a nearest-neighbor model in which the stabilities of iG--iC pairs depend on the adjacent iG--iC or G--C pairs. For 5'-CG-3'/3'-GC-5' and 5'-GG-3'/3'-CC-5' nearest neighbors, the free energy differences upon iG-iC replacement are smaller than 0.2 kcal/mol at 37 degrees C, regardless of the number of replacements. For 5'-GC-3'/3'-CG-5', however, each iG--iC replacement adds 0.6 kcal/mol stabilizing free energy at 37 degrees C. Stacking propensities of iG and iC as unpaired nucleotides at the end of a duplex are similar to those of G and C. An NMR structure is reported for r(CiGCGiCG)(2) and found to belong to the A-form family. The structure has substantial deviations from standard A-form but is similar to published NMR and/or crystal structures for r(CGCGCG)(2) and 2'-O-methyl (CGCGCG)(2). These results provide benchmarks for theoretical calculations aimed at understanding the fundamental physical basis for the thermodynamic stabilities of nucleic acid duplexes. | ||
| - | + | Stability and structure of RNA duplexes containing isoguanosine and isocytidine.,Chen X, Kierzek R, Turner DH J Am Chem Soc. 2001 Feb 21;123(7):1267-74. PMID:11456697<ref>PMID:11456697</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Chen, X.]] | [[Category: Chen, X.]] | ||
[[Category: Kierzek, R.]] | [[Category: Kierzek, R.]] | ||
Revision as of 14:23, 28 September 2014
STRUCTURE OF RNA DUPLEXES (CIGCGICG)2
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Categories: Chen, X. | Kierzek, R. | Turner, D H. | Double helix | Rna
