1n36

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1n36.png|left|200px]]
+
==Structure of the Thermus thermophilus 30S ribosomal subunit in the presence of crystallographically disordered codon and near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position==
 +
<StructureSection load='1n36' size='340' side='right' caption='[[1n36]], [[Resolution|resolution]] 3.65&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1n36]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N36 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1N36 FirstGlance]. <br>
 +
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
 +
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1j5e|1j5e]], [[1fjg|1fjg]], [[1ibl|1ibl]], [[1ibk|1ibk]], [[1ibm|1ibm]], [[1n32|1n32]], [[1n33|1n33]], [[1n34|1n34]]</td></tr>
 +
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n36 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1n36 RCSB], [http://www.ebi.ac.uk/pdbsum/1n36 PDBsum]</span></td></tr>
 +
<table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n36_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity.
-
{{STRUCTURE_1n36| PDB=1n36 | SCENE= }}
+
Selection of tRNA by the ribosome requires a transition from an open to a closed form.,Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V Cell. 2002 Nov 27;111(5):721-32. PMID:12464183<ref>PMID:12464183</ref>
-
===Structure of the Thermus thermophilus 30S ribosomal subunit in the presence of crystallographically disordered codon and near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_12464183}}
+
-
 
+
-
==About this Structure==
+
-
[[1n36]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N36 OCA].
+
==See Also==
==See Also==
-
*[[Ribosomal protein S10|Ribosomal protein S10]]
 
-
*[[Ribosomal protein S11|Ribosomal protein S11]]
 
-
*[[Ribosomal protein S12|Ribosomal protein S12]]
 
-
*[[Ribosomal protein S13|Ribosomal protein S13]]
 
-
*[[Ribosomal protein S14|Ribosomal protein S14]]
 
-
*[[Ribosomal protein S15|Ribosomal protein S15]]
 
-
*[[Ribosomal protein S16|Ribosomal protein S16]]
 
-
*[[Ribosomal protein S17|Ribosomal protein S17]]
 
-
*[[Ribosomal protein S18|Ribosomal protein S18]]
 
-
*[[Ribosomal protein S19|Ribosomal protein S19]]
 
-
*[[Ribosomal protein S2|Ribosomal protein S2]]
 
-
*[[Ribosomal protein S20|Ribosomal protein S20]]
 
-
*[[Ribosomal protein S3|Ribosomal protein S3]]
 
-
*[[Ribosomal protein S4|Ribosomal protein S4]]
 
-
*[[Ribosomal protein S5|Ribosomal protein S5]]
 
-
*[[Ribosomal protein S6|Ribosomal protein S6]]
 
-
*[[Ribosomal protein S7|Ribosomal protein S7]]
 
-
*[[Ribosomal protein S8|Ribosomal protein S8]]
 
-
*[[Ribosomal protein S9|Ribosomal protein S9]]
 
*[[Ribosomal protein THX|Ribosomal protein THX]]
*[[Ribosomal protein THX|Ribosomal protein THX]]
-
*[[Ribosome|Ribosome]]
+
*[[Ribosome 3D structures|Ribosome 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:012464183</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
[[Category: IV, F V.Murphy.]]
[[Category: IV, F V.Murphy.]]

Revision as of 15:47, 28 September 2014

Structure of the Thermus thermophilus 30S ribosomal subunit in the presence of crystallographically disordered codon and near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position

1n36, resolution 3.65Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n36"
Views
Personal tools
Navigation
Toolbox