1fag
From Proteopedia
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| - | [[Image:1fag.gif|left|200px]] | + | [[Image:1fag.gif|left|200px]] |
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| - | '''STRUCTURE OF CYTOCHROME P450''' | + | {{Structure |
| + | |PDB= 1fag |SIZE=350|CAPTION= <scene name='initialview01'>1fag</scene>, resolution 2.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=PAM:PALMITOLEIC ACID'>PAM</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF CYTOCHROME P450''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FAG is a [ | + | 1FAG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FAG OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid., Li H, Poulos TL, Nat Struct Biol. 1997 Feb;4(2):140-6. PMID:[http:// | + | The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid., Li H, Poulos TL, Nat Struct Biol. 1997 Feb;4(2):140-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9033595 9033595] |
[[Category: Bacillus megaterium]] | [[Category: Bacillus megaterium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: monooxygenase]] | [[Category: monooxygenase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:06:37 2008'' |
Revision as of 09:06, 20 March 2008
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| , resolution 2.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Unspecific monooxygenase, with EC number 1.14.14.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF CYTOCHROME P450
Overview
The substrate-bound structures of two cytochrome P450s, P450cam and P450eryF, are known. While these structures reveal important features that control substrate specificity, the problem of how conformational changes allow for substrate entry and product release remains unsolved. The structure of the haem domain of the bacterial fatty acid hydroxylase, P450BM-3, previously was solved in the substrate-free form. Unlike the substrate-bound P450cam and P450eryF structures, the substrate access channel is open in substrate-free P450BM-3. Here we present the X-ray structure of P450BM-3 at 2.7 A bound with a fatty acid substrate, palmitoleic acid. A comparison of the substrate-bound and -free forms reveals major conformational differences and provides the first detailed picture of substrate-induced conformational changes in a P450.
About this Structure
1FAG is a Single protein structure of sequence from Bacillus megaterium. Full crystallographic information is available from OCA.
Reference
The structure of the cytochrome p450BM-3 haem domain complexed with the fatty acid substrate, palmitoleic acid., Li H, Poulos TL, Nat Struct Biol. 1997 Feb;4(2):140-6. PMID:9033595
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