1fb0
From Proteopedia
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| - | [[Image:1fb0.gif|left|200px]] | + | [[Image:1fb0.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF THIOREDOXIN M FROM SPINACH CHLOROPLAST (REDUCED FORM)''' | + | {{Structure |
| + | |PDB= 1fb0 |SIZE=350|CAPTION= <scene name='initialview01'>1fb0</scene>, resolution 2.26Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''CRYSTAL STRUCTURE OF THIOREDOXIN M FROM SPINACH CHLOROPLAST (REDUCED FORM)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FB0 is a [ | + | 1FB0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FB0 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structures of two functionally different thioredoxins in spinach chloroplasts., Capitani G, Markovic-Housley Z, DelVal G, Morris M, Jansonius JN, Schurmann P, J Mol Biol. 2000 Sep 8;302(1):135-54. PMID:[http:// | + | Crystal structures of two functionally different thioredoxins in spinach chloroplasts., Capitani G, Markovic-Housley Z, DelVal G, Morris M, Jansonius JN, Schurmann P, J Mol Biol. 2000 Sep 8;302(1):135-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10964566 10964566] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Spinacia oleracea]] | [[Category: Spinacia oleracea]] | ||
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[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:06:45 2008'' |
Revision as of 09:06, 20 March 2008
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| , resolution 2.26Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THIOREDOXIN M FROM SPINACH CHLOROPLAST (REDUCED FORM)
Overview
Thioredoxins are small ubiquitous proteins which act as general protein disulfide reductases in living cells. Chloroplasts contain two distinct thioredoxins ( f and m) with different phylogenetic origin. Both act as enzyme regulatory proteins but have different specificities towards target enzymes. Thioredoxin f (Trx f), which shares only low sequence identity with thioredoxin m (Trx m) and with all other known thioredoxins, activates enzymes of the Calvin cycle and other photosynthetic processes. Trx m shows high sequence similarity with bacterial thioredoxins and activates other chloroplast enzymes. The here described structural studies of the two chloroplast thioredoxins were carried out in order to gain insight into the structure/function relationships of these proteins. Crystal structures were determined for oxidized, recombinant thioredoxin f (Trx f-L) and at the N terminus truncated form of it (Trx f-S), as well as for oxidized and reduced thioredoxin m (at 2.1 and 2.3 A resolution, respectively). Whereas thioredoxin f crystallized as a monomer, both truncated thioredoxin f and thioredoxin m crystallized as non-covalent dimers. The structures of thioredoxins f and m exhibit the typical thioredoxin fold consisting of a central twisted five-stranded beta-sheet surrounded by four alpha-helices. Thioredoxin f contains an additional alpha-helix at the N terminus and an exposed third cysteine close to the active site. The overall three-dimensional structures of the two chloroplast thioredoxins are quite similar. However, the two proteins have a significantly different surface topology and charge distribution around the active site. An interesting feature which might significantly contribute to the specificity of thioredoxin f is an inherent flexibility of its active site, which has expressed itself crystallographically in two different crystal forms.
About this Structure
1FB0 is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
Crystal structures of two functionally different thioredoxins in spinach chloroplasts., Capitani G, Markovic-Housley Z, DelVal G, Morris M, Jansonius JN, Schurmann P, J Mol Biol. 2000 Sep 8;302(1):135-54. PMID:10964566
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