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2ixs
From Proteopedia
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Revision as of 15:14, 30 October 2007
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STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE
Overview
Rare-cutting restriction enzymes are important tools in genome analysis., We report here the crystal structure of SdaI restriction endonuclease, which is specific for the 8 bp sequence CCTGCA/GG ("/" designates the, cleavage site). Unlike orthodox Type IIP enzymes, which are single domain, proteins, the SdaI monomer is composed of two structural domains. The N, domain contains a classical winged helix-turn-helix (wHTH) DNA binding, motif, while the C domain shows a typical restriction endonuclease fold., The active site of SdaI is located within the C domain and represents a, variant of the canonical PD-(D/E)XK motif. SdaI determinants of sequence, specificity are clustered on the recognition helix of the wHTH motif at, the N domain. The modular architecture of SdaI, wherein one domain, ... [(full description)]
About this Structure
2IXS is a [Single protein] structure of sequence from [Streptomyces diastaticus] with SO4, EPE and TRS as [ligands]. Active as [Type II site-specific deoxyribonuclease], with EC number [3.1.21.4]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture., Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V, Structure. 2006 Sep;14(9):1389-400. PMID:16962970
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