1up8
From Proteopedia
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| - | [[ | + | ==RECOMBINANT VANADIUM-DEPENDENT BROMOPEROXIDASE FROM RED ALGAE CORALLINA PILULIFERA== |
| + | <StructureSection load='1up8' size='340' side='right' caption='[[1up8]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1up8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Corallina_pilulifera Corallina pilulifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UP8 FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1up8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1up8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1up8 RCSB], [http://www.ebi.ac.uk/pdbsum/1up8 PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/up/1up8_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bromoperoxidase from the macro-alga Corallina pilulifera is an enzyme that possesses vanadate in the catalytic center, and shows a significant thermostability and stability toward organic solvents. The structural analysis of the recombinant enzyme overexpressed in yeast revealed that it contains one calcium atom per subunit. This has been confirmed by inductively coupled plasma emission spectrometry experiments. The study of the effect of metal ions on the apo-enzyme stability has shown that the calcium ion significantly increased the enzyme stability. In addition, vanadate also increased the thermostability and strontium and magnesium ions had similar effects as calcium. The holo-enzyme shows high stability in a range of organic solvents. The effect of the different ions and solvents on the structure of the enzyme has been studied by circular dichroism experiments. The high stability of the enzyme in the presence of organic solvents is useful for its application as a biocatalyst. | ||
| - | + | Enhancing effect of calcium and vanadium ions on thermal stability of bromoperoxidase from Corallina pilulifera.,Garcia-Rodriguez E, Ohshiro T, Aibara T, Izumi Y, Littlechild J J Biol Inorg Chem. 2005 May;10(3):275-82. Epub 2005 Mar 18. PMID:15776268<ref>PMID:15776268</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Haloperoxidase|Haloperoxidase]] | *[[Haloperoxidase|Haloperoxidase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Corallina pilulifera]] | [[Category: Corallina pilulifera]] | ||
[[Category: Garcia-Rodriguez, E.]] | [[Category: Garcia-Rodriguez, E.]] | ||
Revision as of 20:02, 28 September 2014
RECOMBINANT VANADIUM-DEPENDENT BROMOPEROXIDASE FROM RED ALGAE CORALLINA PILULIFERA
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