1fo4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1fo4.gif|left|200px]]<br /><applet load="1fo4" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1fo4.gif|left|200px]]
-
caption="1fo4, resolution 2.1&Aring;" />
+
 
-
'''CRYSTAL STRUCTURE OF XANTHINE DEHYDROGENASE ISOLATED FROM BOVINE MILK'''<br />
+
{{Structure
 +
|PDB= 1fo4 |SIZE=350|CAPTION= <scene name='initialview01'>1fo4</scene>, resolution 2.1&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=MTE:PHOSPHONIC+ACIDMONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,7,8A,9,10,10A-HEXAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)ESTER'>MTE</scene>, <scene name='pdbligand=MOS:DIOXOTHIOMOLYBDENUM(VI)+ION'>MOS</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Xanthine_dehydrogenase Xanthine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.4 1.17.1.4]
 +
|GENE=
 +
}}
 +
 
 +
'''CRYSTAL STRUCTURE OF XANTHINE DEHYDROGENASE ISOLATED FROM BOVINE MILK'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1FO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=FES:'>FES</scene>, <scene name='pdbligand=MTE:'>MTE</scene>, <scene name='pdbligand=MOS:'>MOS</scene>, <scene name='pdbligand=SAL:'>SAL</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Xanthine_dehydrogenase Xanthine dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.1.4 1.17.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FO4 OCA].
+
1FO4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FO4 OCA].
==Reference==
==Reference==
-
Crystal structures of bovine milk xanthine dehydrogenase and xanthine oxidase: structure-based mechanism of conversion., Enroth C, Eger BT, Okamoto K, Nishino T, Nishino T, Pai EF, Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10723-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11005854 11005854]
+
Crystal structures of bovine milk xanthine dehydrogenase and xanthine oxidase: structure-based mechanism of conversion., Enroth C, Eger BT, Okamoto K, Nishino T, Nishino T, Pai EF, Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10723-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11005854 11005854]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 26: Line 35:
[[Category: MTE]]
[[Category: MTE]]
[[Category: SAL]]
[[Category: SAL]]
-
[[Category: 2fe-2s iron sulfur centers]]
+
[[Category: 2fe-2s iron sulfur center]]
[[Category: fad]]
[[Category: fad]]
[[Category: molybdopterin]]
[[Category: molybdopterin]]
Line 32: Line 41:
[[Category: xanthine dehydrogenase]]
[[Category: xanthine dehydrogenase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:40:44 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:11:42 2008''

Revision as of 09:11, 20 March 2008

Image:1fo4.gif


PDB ID 1fo4

Drag the structure with the mouse to rotate
, resolution 2.1Å
Ligands: , , , , , and
Activity: Xanthine dehydrogenase, with EC number 1.17.1.4
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF XANTHINE DEHYDROGENASE ISOLATED FROM BOVINE MILK


Overview

Mammalian xanthine oxidoreductases, which catalyze the last two steps in the formation of urate, are synthesized as the dehydrogenase form xanthine dehydrogenase (XDH) but can be readily converted to the oxidase form xanthine oxidase (XO) by oxidation of sulfhydryl residues or by proteolysis. Here, we present the crystal structure of the dimeric (M(r), 290,000) bovine milk XDH at 2.1-A resolution and XO at 2.5-A resolution and describe the major changes that occur on the proteolytic transformation of XDH to the XO form. Each molecule is composed of an N-terminal 20-kDa domain containing two iron sulfur centers, a central 40-kDa flavin adenine dinucleotide domain, and a C-terminal 85-kDa molybdopterin-binding domain with the four redox centers aligned in an almost linear fashion. Cleavage of surface-exposed loops of XDH causes major structural rearrangement of another loop close to the flavin ring (Gln 423Lys 433). This movement partially blocks access of the NAD substrate to the flavin adenine dinucleotide cofactor and changes the electrostatic environment of the active site, reflecting the switch of substrate specificity observed for the two forms of this enzyme.

About this Structure

1FO4 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Crystal structures of bovine milk xanthine dehydrogenase and xanthine oxidase: structure-based mechanism of conversion., Enroth C, Eger BT, Okamoto K, Nishino T, Nishino T, Pai EF, Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10723-8. PMID:11005854

Page seeded by OCA on Thu Mar 20 11:11:42 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fo4"
Personal tools