1w96

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[[Image:1w96.png|left|200px]]
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==CRYSTAL STRUCTURE OF BIOTIN CARBOXYLASE DOMAIN OF ACETYL-COENZYME A CARBOXYLASE FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH SORAPHEN A==
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<StructureSection load='1w96' size='340' side='right' caption='[[1w96]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1w96]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W96 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=S1A:SORAPHEN+A'>S1A</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1od2|1od2]], [[1od4|1od4]], [[1uyr|1uyr]], [[1uys|1uys]], [[1uyt|1uyt]], [[1uyv|1uyv]], [[1w2x|1w2x]], [[1w93|1w93]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetyl-CoA_carboxylase Acetyl-CoA carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.4.1.2 6.4.1.2] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w96 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w96 RCSB], [http://www.ebi.ac.uk/pdbsum/1w96 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/1w96_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Acetyl-coenzyme A carboxylases (ACCs) have crucial roles in fatty acid metabolism. Soraphen A, a macrocyclic polyketide natural product, is a nanomolar inhibitor against the biotin carboxylase (BC) domain of human, yeast, and other eukaryotic ACCs. Here we report the crystal structures of the yeast BC domain, alone and in complex with soraphen A. Soraphen has extensive interactions with an allosteric site, about 25 A from the active site. The specificity of soraphen is explained by large structural differences between the eukaryotic and prokaryotic BC in its binding site, confirmed by our studies on the effects of single-site mutations in this binding site. Unexpectedly, our structures suggest that soraphen may bind in the BC dimer interface and inhibit the BC activity by disrupting the oligomerization of this domain. Observations from native gel electrophoresis confirm this structural insight. The structural information provides a foundation for structure-based design of new inhibitors against these enzymes.
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{{STRUCTURE_1w96| PDB=1w96 | SCENE= }}
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A mechanism for the potent inhibition of eukaryotic acetyl-coenzyme A carboxylase by soraphen A, a macrocyclic polyketide natural product.,Shen Y, Volrath SL, Weatherly SC, Elich TD, Tong L Mol Cell. 2004 Dec 22;16(6):881-91. PMID:15610732<ref>PMID:15610732</ref>
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===CRYSTAL STRUCTURE OF BIOTIN CARBOXYLASE DOMAIN OF ACETYL-COENZYME A CARBOXYLASE FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH SORAPHEN A===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_15610732}}
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==About this Structure==
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[[1w96]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W96 OCA].
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==See Also==
==See Also==
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*[[Acetyl-CoA carboxylase|Acetyl-CoA carboxylase]]
*[[Biotin carboxylase|Biotin carboxylase]]
*[[Biotin carboxylase|Biotin carboxylase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015610732</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Acetyl-CoA carboxylase]]
[[Category: Acetyl-CoA carboxylase]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]

Revision as of 22:31, 28 September 2014

CRYSTAL STRUCTURE OF BIOTIN CARBOXYLASE DOMAIN OF ACETYL-COENZYME A CARBOXYLASE FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH SORAPHEN A

1w96, resolution 1.80Å

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