1fwi

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[[Image:1fwi.gif|left|200px]]<br /><applet load="1fwi" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1fwi.gif|left|200px]]
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caption="1fwi, resolution 2.0&Aring;" />
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'''KLEBSIELLA AEROGENES UREASE, H134A VARIANT'''<br />
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{{Structure
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|PDB= 1fwi |SIZE=350|CAPTION= <scene name='initialview01'>1fwi</scene>, resolution 2.0&Aring;
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|SITE= <scene name='pdbsite=ACT:Residue+Implicated+In+Catalysis'>ACT</scene> and <scene name='pdbsite=NIL:Ni+Metallocenter'>NIL</scene>
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|LIGAND= <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5]
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|GENE=
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}}
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'''KLEBSIELLA AEROGENES UREASE, H134A VARIANT'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1FWI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] Known structural/functional Sites: <scene name='pdbsite=ACT:Residue+Implicated+In+Catalysis'>ACT</scene> and <scene name='pdbsite=NIL:Ni+Metallocenter'>NIL</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FWI OCA].
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1FWI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FWI OCA].
==Reference==
==Reference==
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Characterization of the mononickel metallocenter in H134A mutant urease., Park IS, Michel LO, Pearson MA, Jabri E, Karplus PA, Wang S, Dong J, Scott RA, Koehler BP, Johnson MK, Hausinger RP, J Biol Chem. 1996 Aug 2;271(31):18632-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8702515 8702515]
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Characterization of the mononickel metallocenter in H134A mutant urease., Park IS, Michel LO, Pearson MA, Jabri E, Karplus PA, Wang S, Dong J, Scott RA, Koehler BP, Johnson MK, Hausinger RP, J Biol Chem. 1996 Aug 2;271(31):18632-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8702515 8702515]
[[Category: Klebsiella aerogenes]]
[[Category: Klebsiella aerogenes]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: nickel metalloenzyme]]
[[Category: nickel metalloenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:43:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:14:55 2008''

Revision as of 09:14, 20 March 2008

Image:1fwi.gif


PDB ID 1fwi

Drag the structure with the mouse to rotate
, resolution 2.0Å
Sites: and
Ligands:
Activity: Urease, with EC number 3.5.1.5
Coordinates: save as pdb, mmCIF, xml



KLEBSIELLA AEROGENES UREASE, H134A VARIANT


Overview

A mutant form of Klebsiella aerogenes urease possessing Ala instead of His at position 134 (H134A) is inactive and binds approximately half the normal complement of nickel (Park, I.-S., and Hausinger, R. P.(1993) Protein Sci. 2, 1034-1041). The crystal structure of the H134A protein was obtained at 2.0-A resolution, and it confirms that only Ni-1 of the two nickel ions found in the native enzyme is present. In contrast to the pseudotetrahedral geometry observed for Ni-1 in native urease (where it is liganded by His-246, His-272, one oxygen atom of carbamylated Lys-217, and a water molecule at partial occupancy), the mononickel metallocenter in the H134A protein was found to possess octahedral geometry and was coordinated by the above protein ligands plus three water molecules. The nickel site of H134A urease was probed by UV-visible, variable temperature magnetic circular dichroism, and x-ray absorption spectroscopies. The spectroscopic data are consistent with the presence of Ni(II) in octahedral geometry coordinated by two histidylimidazoles and additional oxygen and/or nitrogen donors. These data underscore the requirement of Ni-2 for formation of active urease and demonstrate the important role of Ni-2 in establishing the proper Ni-1 coordination geometry.

About this Structure

1FWI is a Protein complex structure of sequences from Klebsiella aerogenes. Full crystallographic information is available from OCA.

Reference

Characterization of the mononickel metallocenter in H134A mutant urease., Park IS, Michel LO, Pearson MA, Jabri E, Karplus PA, Wang S, Dong J, Scott RA, Koehler BP, Johnson MK, Hausinger RP, J Biol Chem. 1996 Aug 2;271(31):18632-7. PMID:8702515

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