1fx5

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[[Image:1fx5.gif|left|200px]]<br /><applet load="1fx5" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1fx5.gif|left|200px]]
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caption="1fx5, resolution 2.2&Aring;" />
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'''CRYSTAL STRUCTURE ANALYSIS OF ULEX EUROPAEUS LECTIN I'''<br />
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{{Structure
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|PDB= 1fx5 |SIZE=350|CAPTION= <scene name='initialview01'>1fx5</scene>, resolution 2.2&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE ANALYSIS OF ULEX EUROPAEUS LECTIN I'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1FX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ulex_europaeus Ulex europaeus] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MRD:'>MRD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX5 OCA].
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1FX5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ulex_europaeus Ulex europaeus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FX5 OCA].
==Reference==
==Reference==
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The 2.2 A resolution structure of the O(H) blood-group-specific lectin I from Ulex europaeus., Audette GF, Vandonselaar M, Delbaere LT, J Mol Biol. 2000 Dec 1;304(3):423-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11090284 11090284]
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The 2.2 A resolution structure of the O(H) blood-group-specific lectin I from Ulex europaeus., Audette GF, Vandonselaar M, Delbaere LT, J Mol Biol. 2000 Dec 1;304(3):423-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11090284 11090284]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Ulex europaeus]]
[[Category: Ulex europaeus]]
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[[Category: ue-i]]
[[Category: ue-i]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:43:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:15:11 2008''

Revision as of 09:15, 20 March 2008

Image:1fx5.gif


PDB ID 1fx5

Drag the structure with the mouse to rotate
, resolution 2.2Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE ANALYSIS OF ULEX EUROPAEUS LECTIN I


Overview

The tertiary and quaternary structure of the lectin I from Ulex europaeus (UE-I) has been determined to 2.2 A resolution. UE-I is a dimeric metalloglycoprotein that binds the H-type 2 human blood group determinant [alpha-L-Fucalpha(1-->2)-beta-D-Galbeta(1-->4)-beta-D-Glc NAcalpha-]. Nine changes from the published amino acid sequence were necessary to account for the electron density. The quaternary structural organization of UE-I is that of the most commonly occurring legume lectin dimer. The tertiary structure of the monomeric subunits is similar to that in the conventional lectin subunit; however, some structural differences are noted. These differences include a four-stranded anti-parallel "S" sheet in UE-I versus the five-stranded S sheet in other lectin monomers. The Ala residue of the Ala-Asp cis-peptide bond present in the carbohydrate-binding site of the conventional lectin monomer is replaced with a Thr in the UE-I structure. Also, a novel disulfide bridge linking Cys115 and Cys150 is present. There are two metallic ions, one calcium and the other manganese, per subunit. N-linked oligosaccharides are at residues 23 and 111 of each subunit. One molecule of R-2-methyl-2, 4-pentanediol (R-MPD) is present in a shallow depression on the surface of each subunit. In order to examine the binding of the H-type 2 blood group determinant by UE-I, its beta-methyl glycoside (H-type 2-OMe) was docked into the binding site of R-MPD. The epitope previously identified for H-type 2-OMe by chemical mapping proved, with only minor adjustment of amino acid residues, to be complementary to the shallow cavity occupied by R-MPD in the structure. Several key interactions have been proposed between the H-type 2-OMe and UE-I.

About this Structure

1FX5 is a Single protein structure of sequence from Ulex europaeus. Full crystallographic information is available from OCA.

Reference

The 2.2 A resolution structure of the O(H) blood-group-specific lectin I from Ulex europaeus., Audette GF, Vandonselaar M, Delbaere LT, J Mol Biol. 2000 Dec 1;304(3):423-33. PMID:11090284

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