2c4b

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[[Image:2c4b.png|left|200px]]
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==INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A==
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<StructureSection load='2c4b' size='340' side='right' caption='[[2c4b]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2c4b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C4B FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1a2p|1a2p]], [[1b20|1b20]], [[1b21|1b21]], [[1b27|1b27]], [[1b2s|1b2s]], [[1b2u|1b2u]], [[1b2x|1b2x]], [[1b2z|1b2z]], [[1b3s|1b3s]], [[1ban|1ban]], [[1bao|1bao]], [[1bgs|1bgs]], [[1bne|1bne]], [[1bnf|1bnf]], [[1bng|1bng]], [[1bni|1bni]], [[1bnj|1bnj]], [[1bnr|1bnr]], [[1bns|1bns]], [[1brg|1brg]], [[1brh|1brh]], [[1bri|1bri]], [[1brj|1brj]], [[1brk|1brk]], [[1brn|1brn]], [[1brs|1brs]], [[1bsa|1bsa]], [[1bsb|1bsb]], [[1bsc|1bsc]], [[1bsd|1bsd]], [[1bse|1bse]], [[1fw7|1fw7]], [[1h9h|1h9h]], [[1h9i|1h9i]], [[1ha9|1ha9]], [[1ib9|1ib9]], [[1rnb|1rnb]], [[1w7z|1w7z]], [[1x1u|1x1u]], [[1x1w|1x1w]], [[1x1x|1x1x]], [[1x1y|1x1y]], [[1yvs|1yvs]], [[2eti|2eti]], [[2let|2let]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2c4b RCSB], [http://www.ebi.ac.uk/pdbsum/2c4b PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c4/2c4b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins.
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{{STRUCTURE_2c4b| PDB=2c4b | SCENE= }}
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Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI.,Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652<ref>PMID:16337652</ref>
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===INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_16337652}}
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==About this Structure==
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[[2c4b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA].
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==See Also==
==See Also==
*[[Barnase|Barnase]]
*[[Barnase|Barnase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016337652</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Bacillus amyloliquefaciens]]
[[Category: Bacillus amyloliquefaciens]]
[[Category: Heinz, D W.]]
[[Category: Heinz, D W.]]

Revision as of 03:01, 29 September 2014

INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A

2c4b, resolution 1.30Å

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