1fyl
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1fyl.gif|left|200px]] | + | [[Image:1fyl.gif|left|200px]] |
| - | + | ||
| - | '''SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA IN A HEAD-TO-HEAD ORIENTATION''' | + | {{Structure |
| + | |PDB= 1fyl |SIZE=350|CAPTION= <scene name='initialview01'>1fyl</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BR:BROMIDE ION'>BR</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA IN A HEAD-TO-HEAD ORIENTATION''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1FYL is a [ | + | 1FYL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYL OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal packing interaction that blocks crystallization of a site-specific DNA binding protein-DNA complex., Littlefield O, Nelson HC, Proteins. 2001 Nov 15;45(3):219-28. PMID:[http:// | + | Crystal packing interaction that blocks crystallization of a site-specific DNA binding protein-DNA complex., Littlefield O, Nelson HC, Proteins. 2001 Nov 15;45(3):219-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11599025 11599025] |
[[Category: Kluyveromyces lactis]] | [[Category: Kluyveromyces lactis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: static disorder]] | [[Category: static disorder]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:15:42 2008'' |
Revision as of 09:15, 20 March 2008
| |||||||
| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA IN A HEAD-TO-HEAD ORIENTATION
Overview
We present here three high-resolution crystal structures of complexes between the DNA-binding domain of the heat-shock transcription factor (HSF) and DNA oligomers. Although the DNA oligomers contain HSF's specific binding sequence, called a heat-shock element, the crystal structures do not contain the specific protein-DNA complex. In one crystal structure, the 10 base pair DNA oligomer is statically disordered. In the other two related structures, the 12 base pair DNA oligomers are in unique positions, but the protein-DNA contacts in these two crystals are not sequence specific. In all three structures, the DNA appears to act as a rigid, polyanion scaffold to support columns of proteins in a crystalline lattice. A robust crystal packing interface between protein monomers obscures the true DNA-binding surface, known from previous genetic and biochemical studies. By redesigning the protein to interfere with the crystal lattice contacts, we were able to obtain physiologically relevant crystals in a specific protein-DNA complex. Thus, a crystal-packing interface was able to prevent the weak, but physiological relevant interactions between a protein and DNA.
About this Structure
1FYL is a Single protein structure of sequence from Kluyveromyces lactis. Full crystallographic information is available from OCA.
Reference
Crystal packing interaction that blocks crystallization of a site-specific DNA binding protein-DNA complex., Littlefield O, Nelson HC, Proteins. 2001 Nov 15;45(3):219-28. PMID:11599025
Page seeded by OCA on Thu Mar 20 11:15:42 2008
