2l9w
From Proteopedia
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| - | [[ | + | ==Solution Structure of the C-terminal domain of Prp24== |
| + | <StructureSection load='2l9w' size='340' side='right' caption='[[2l9w]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2l9w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L9W FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2go9|2go9]], [[2ghp|2ghp]], [[2kh9|2kh9]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRP24, YM8156.10C, YMR268C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l9w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2l9w RCSB], [http://www.ebi.ac.uk/pdbsum/2l9w PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The essential splicing factor Prp24 contains four RNA Recognition Motif (RRM) domains, and functions to anneal U6 and U4 RNAs during spliceosome assembly. Here, we report the structure and characterization of the C-terminal RRM4. This domain adopts a novel non-canonical RRM fold with two additional flanking alpha-helices that occlude its beta-sheet face, forming an occluded RRM (oRRM) domain. The flanking helices form a large electropositive surface. oRRM4 binds to and unwinds the U6 internal stem loop (U6 ISL), a stable helix that must be unwound during U4/U6 assembly. NMR data indicate that the process starts with the terminal base pairs of the helix and proceeds toward the loop. We propose a mechanistic and structural model of Prp24's annealing activity in which oRRM4 functions to destabilize the U6 ISL during U4/U6 assembly. | ||
| - | + | A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop.,Martin-Tumasz S, Richie AC, Clos LJ 2nd, Brow DA, Butcher SE Nucleic Acids Res. 2011 Jun 7. PMID:21653550<ref>PMID:21653550</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
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==See Also== | ==See Also== | ||
*[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]] | *[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | < | + | __TOC__ |
| + | </StructureSection> | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Butcher, S E.]] | [[Category: Butcher, S E.]] | ||
Revision as of 03:30, 29 September 2014
Solution Structure of the C-terminal domain of Prp24
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