1g88

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[[Image:1g88.gif|left|200px]]<br /><applet load="1g88" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1g88.gif|left|200px]]
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caption="1g88, resolution 3.00&Aring;" />
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'''S4AFL3ARG515 MUTANT'''<br />
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{{Structure
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|PDB= 1g88 |SIZE=350|CAPTION= <scene name='initialview01'>1g88</scene>, resolution 3.00&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''S4AFL3ARG515 MUTANT'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1G88 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G88 OCA].
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1G88 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G88 OCA].
==Reference==
==Reference==
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The L3 loop and C-terminal phosphorylation jointly define Smad protein trimerization., Chacko BM, Qin B, Correia JJ, Lam SS, de Caestecker MP, Lin K, Nat Struct Biol. 2001 Mar;8(3):248-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11224571 11224571]
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The L3 loop and C-terminal phosphorylation jointly define Smad protein trimerization., Chacko BM, Qin B, Correia JJ, Lam SS, de Caestecker MP, Lin K, Nat Struct Biol. 2001 Mar;8(3):248-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11224571 11224571]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transcriptional factor]]
[[Category: transcriptional factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:47:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:19:34 2008''

Revision as of 09:19, 20 March 2008


PDB ID 1g88

Drag the structure with the mouse to rotate
, resolution 3.00Å
Coordinates: save as pdb, mmCIF, xml



S4AFL3ARG515 MUTANT


Contents

Overview

Smad proteins mediate the transforming growth factor beta responses. C-terminal phosphorylation of R-Smads leads to the recruitment of Smad4 and the formation of active signaling complexes. We investigated the mechanism of phosphorylation-induced Smad complex formation with an activating pseudo-phosphorylated Smad3. Pseudo-phosphorylated Smad3 has a greater propensity to homotrimerize, and recruits Smad4 to form a heterotrimer containing two Smad3 and one Smad4. The trimeric interaction is mediated through conserved interfaces to which tumorigenic mutations map. Furthermore, a conserved Arg residue within the L3 loop, located near the C-terminal phosphorylation sites of the neighboring subunit, is essential for trimerization. We propose that the phosphorylated C-terminal residues interact with the L3 loop of the neighboring subunit to stabilize the trimer interaction.

Disease

Known diseases associated with this structure: Juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome OMIM:[600993], Pancreatic cancer OMIM:[600993], Polyposis, juvenile intestinal OMIM:[600993]

About this Structure

1G88 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The L3 loop and C-terminal phosphorylation jointly define Smad protein trimerization., Chacko BM, Qin B, Correia JJ, Lam SS, de Caestecker MP, Lin K, Nat Struct Biol. 2001 Mar;8(3):248-53. PMID:11224571

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