1zxx

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[[Image:1zxx.png|left|200px]]
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==The crystal structure of phosphofructokinase from Lactobacillus delbrueckii==
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<StructureSection load='1zxx' size='340' side='right' caption='[[1zxx]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1zxx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_delbrueckii_subsp._bulgaricus Lactobacillus delbrueckii subsp. bulgaricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZXX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZXX FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pfkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1585 Lactobacillus delbrueckii subsp. bulgaricus])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phosphofructokinase 6-phosphofructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.11 2.7.1.11] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zxx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1zxx RCSB], [http://www.ebi.ac.uk/pdbsum/1zxx PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zx/1zxx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phosphofructokinase from Lactobacillus delbrueckii subspecies bulgaricus (LbPFK) has been reported to be a nonallosteric analogue of phosphofructokinase from Escherichia coli at pH 8.2 [Le Bras et al. (1991) Eur. J. Biochem. 198, 683-687]. A reexamination of the kinetics of this enzyme shows LbPFK to have limited binding affinity toward the allosteric ligands, MgADP and PEP, with dissociation constants of approximately 20 mM for both. Their allosteric effects are observed only at high concentrations of these ligands, with both exhibiting inhibitory effects on substrate binding. No pH dependence was observed for the binding and the influence of MgADP and PEP on the enzyme. To attempt to explain these results, the crystal structure of LbPFK was solved using molecular replacement to 1.86 A resolution. A comparative study of the LbPFK structure with that of phosphofructokinases from E. coli (EcPFK) and Bacillus stearothermophilus (BsPFK) reveals a structure with conserved fold and substrate binding site. The effector binding site, however, shows many differences that could explain the observed decreases in binding affinity for MgADP and PEP in LbPFK as compared to the other two enzymes.
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{{STRUCTURE_1zxx| PDB=1zxx | SCENE= }}
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Kinetic and structural characterization of phosphofructokinase from Lactobacillus bulgaricus.,Paricharttanakul NM, Ye S, Menefee AL, Javid-Majd F, Sacchettini JC, Reinhart GD Biochemistry. 2005 Nov 22;44(46):15280-6. PMID:16285731<ref>PMID:16285731</ref>
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===The crystal structure of phosphofructokinase from Lactobacillus delbrueckii===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_16285731}}
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==About this Structure==
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[[1zxx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_delbrueckii_subsp._bulgaricus Lactobacillus delbrueckii subsp. bulgaricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZXX OCA].
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==See Also==
==See Also==
*[[Phosphofructokinase (PFK)|Phosphofructokinase (PFK)]]
*[[Phosphofructokinase (PFK)|Phosphofructokinase (PFK)]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:016285731</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: 6-phosphofructokinase]]
[[Category: 6-phosphofructokinase]]
[[Category: Lactobacillus delbrueckii subsp. bulgaricus]]
[[Category: Lactobacillus delbrueckii subsp. bulgaricus]]

Revision as of 05:56, 29 September 2014

The crystal structure of phosphofructokinase from Lactobacillus delbrueckii

1zxx, resolution 1.85Å

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