1g8j

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1g8j.jpg|left|200px]]<br /><applet load="1g8j" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1g8j.jpg|left|200px]]
-
caption="1g8j, resolution 2.03&Aring;" />
+
 
-
'''CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS'''<br />
+
{{Structure
 +
|PDB= 1g8j |SIZE=350|CAPTION= <scene name='initialview01'>1g8j</scene>, resolution 2.03&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene>, <scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene> and <scene name='pdbligand=FES:FE2/S2 (INORGANIC) CLUSTER'>FES</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1G8J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=MGD:'>MGD</scene>, <scene name='pdbligand=O:'>O</scene>, <scene name='pdbligand=4MO:'>4MO</scene>, <scene name='pdbligand=F3S:'>F3S</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8J OCA].
+
1G8J is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8J OCA].
==Reference==
==Reference==
-
Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A., Ellis PJ, Conrads T, Hille R, Kuhn P, Structure. 2001 Feb 7;9(2):125-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11250197 11250197]
+
Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A., Ellis PJ, Conrads T, Hille R, Kuhn P, Structure. 2001 Feb 7;9(2):125-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11250197 11250197]
[[Category: Alcaligenes faecalis]]
[[Category: Alcaligenes faecalis]]
[[Category: Protein complex]]
[[Category: Protein complex]]
Line 29: Line 38:
[[Category: rieske]]
[[Category: rieske]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:47:19 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:19:43 2008''

Revision as of 09:19, 20 March 2008

Image:1g8j.jpg


PDB ID 1g8j

Drag the structure with the mouse to rotate
, resolution 2.03Å
Ligands: , , , and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS


Overview

BACKGROUND: Arsenite oxidase from Alcaligenes faecalis NCIB 8687 is a molybdenum/iron protein involved in the detoxification of arsenic. It is induced by the presence of AsO(2-) (arsenite) and functions to oxidize As(III)O(2-), which binds to essential sulfhydryl groups of proteins and dithiols, to the relatively less toxic As(V)O(4)(3-) (arsenate) prior to methylation. RESULTS: Using a combination of multiple isomorphous replacement with anomalous scattering (MIRAS) and multiple-wavelength anomalous dispersion (MAD) methods, the crystal structure of arsenite oxidase was determined to 2.03 A in a P2(1) crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. Arsenite oxidase consists of a large subunit of 825 residues and a small subunit of approximately 134 residues. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site. CONCLUSIONS: The large subunit of arsenite oxidase is similar to other members of the dimethylsulfoxide (DMSO) reductase family of molybdenum enzymes, particularly the dissimilatory periplasmic nitrate reductase from Desulfovibrio desulfuricans, but is unique in having no covalent bond between the polypeptide and the Mo atom. The small subunit has no counterpart among known Mo protein structures but is homologous to the Rieske [2Fe-2S] protein domain of the cytochrome bc(1) and cytochrome b(6)f complexes and to the Rieske domain of naphthalene 1,2-dioxygenase.

About this Structure

1G8J is a Protein complex structure of sequences from Alcaligenes faecalis. Full crystallographic information is available from OCA.

Reference

Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A., Ellis PJ, Conrads T, Hille R, Kuhn P, Structure. 2001 Feb 7;9(2):125-32. PMID:11250197[[Category: [2fe-2s] cluster]] [[Category: [3fe-4s] cluster]]

Page seeded by OCA on Thu Mar 20 11:19:43 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g8j"
Personal tools