1g8w

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[[Image:1g8w.gif|left|200px]]<br /><applet load="1g8w" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1g8w.gif|left|200px]]
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caption="1g8w, resolution 2.8&Aring;" />
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'''IMPROVED STRUCTURE OF PHYTOHEMAGGLUTININ-L FROM THE KIDNEY BEAN'''<br />
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{{Structure
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|PDB= 1g8w |SIZE=350|CAPTION= <scene name='initialview01'>1g8w</scene>, resolution 2.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''IMPROVED STRUCTURE OF PHYTOHEMAGGLUTININ-L FROM THE KIDNEY BEAN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1G8W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8W OCA].
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1G8W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8W OCA].
==Reference==
==Reference==
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Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus., Buts L, Dao-Thi MH, Loris R, Wyns L, Etzler M, Hamelryck T, J Mol Biol. 2001 May 25;309(1):193-201. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11491289 11491289]
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Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus., Buts L, Dao-Thi MH, Loris R, Wyns L, Etzler M, Hamelryck T, J Mol Biol. 2001 May 25;309(1):193-201. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11491289 11491289]
[[Category: Phaseolus vulgaris]]
[[Category: Phaseolus vulgaris]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: legume lectin]]
[[Category: legume lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:47:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:19:52 2008''

Revision as of 09:19, 20 March 2008

Image:1g8w.gif


PDB ID 1g8w

Drag the structure with the mouse to rotate
, resolution 2.8Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



IMPROVED STRUCTURE OF PHYTOHEMAGGLUTININ-L FROM THE KIDNEY BEAN


Overview

The legume lectins are widely used as a model system for studying protein-carbohydrate and protein-protein interactions. They exhibit a fascinating quaternary structure variation, which becomes important when they interact with multivalent glycoconjugates, for instance those on cell surfaces. Recently, it has become clear that certain lectins form weakly associated oligomers. This phenomenon may play a role in the regulation of receptor crosslinking and subsequent signal transduction. The crystal structure of DB58, a dimeric lectin from the legume Dolichos biflorus reveals a separate dimer of a previously unobserved type, in addition to a tetramer consisting of two such dimers. This tetramer resembles that formed by DBL, the seed lectin from the same plant. A single amino acid substitution in DB58 affects the conformation and flexibility of a loop in the canonical dimer interface. This disrupts the formation of a stable DBL-like tetramer in solution, but does not prohibit its formation in suitable conditions, which greatly increases the possibilities for the cross-linking of multivalent ligands. The non-canonical DB58 dimer has a buried symmetrical alpha helix, which can be present in the crystal in either of two antiparallel orientations. Two existing structures and datasets for lectins with similar quaternary structures were reconsidered. A central alpha helix could be observed in the soybean lectin, but not in the leucoagglutinating lectin from Phaseolus vulgaris. The relative position and orientation of the carbohydrate-binding sites in the DB58 dimer may affect its ability to crosslink mulitivalent ligands, compared to the other legume lectin dimers.

About this Structure

1G8W is a Single protein structure of sequence from Phaseolus vulgaris. Full crystallographic information is available from OCA.

Reference

Weak protein-protein interactions in lectins: the crystal structure of a vegetative lectin from the legume Dolichos biflorus., Buts L, Dao-Thi MH, Loris R, Wyns L, Etzler M, Hamelryck T, J Mol Biol. 2001 May 25;309(1):193-201. PMID:11491289

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