1gcy
From Proteopedia
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| - | [[Image:1gcy.gif|left|200px]] | + | [[Image:1gcy.gif|left|200px]] |
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| - | '''HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE''' | + | {{Structure |
| + | |PDB= 1gcy |SIZE=350|CAPTION= <scene name='initialview01'>1gcy</scene>, resolution 1.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Glucan_1,4-alpha-maltotetraohydrolase Glucan 1,4-alpha-maltotetraohydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.60 3.2.1.60] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GCY is a [ | + | 1GCY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCY OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallization and structural analysis of intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri., Mezaki Y, Katsuya Y, Kubota M, Matsuura Y, Biosci Biotechnol Biochem. 2001 Jan;65(1):222-5. PMID:[http:// | + | Crystallization and structural analysis of intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri., Mezaki Y, Katsuya Y, Kubota M, Matsuura Y, Biosci Biotechnol Biochem. 2001 Jan;65(1):222-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11272837 11272837] |
[[Category: Glucan 1,4-alpha-maltotetraohydrolase]] | [[Category: Glucan 1,4-alpha-maltotetraohydrolase]] | ||
[[Category: Pseudomonas stutzeri]] | [[Category: Pseudomonas stutzeri]] | ||
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[[Category: beta-alpha-barrel]] | [[Category: beta-alpha-barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:21:28 2008'' |
Revision as of 09:21, 20 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Glucan 1,4-alpha-maltotetraohydrolase, with EC number 3.2.1.60 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE
Overview
The intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri (G4-1), which has a raw starch binding domain, has been crystallized. The structure was identified (PDB entry 1GCY) by the molecular replacement method using the structure of its catalytic domain (G4-2). The result showed that the raw starch binding domain is in a disordered state, the corresponding electron densities being almost invisible. Superposition of these two enzyme forms showed evidence for the possible location of the raw starch binding domain (SBD). This crystal is a novel case, in that it forms a regular lattice incorporating flexibly bound SBD in the channel of crystal packing of the catalytic domains.
About this Structure
1GCY is a Single protein structure of sequence from Pseudomonas stutzeri. Full crystallographic information is available from OCA.
Reference
Crystallization and structural analysis of intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri., Mezaki Y, Katsuya Y, Kubota M, Matsuura Y, Biosci Biotechnol Biochem. 2001 Jan;65(1):222-5. PMID:11272837
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