2qow

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[[Image:2qow.png|left|200px]]
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==Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.==
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<StructureSection load='2qow' size='340' side='right' caption='[[2qow]], [[Resolution|resolution]] 3.93&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2qow]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QOW FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SCM:SPECTINOMYCIN'>SCM</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qou|2qou]], [[2qov|2qov]], [[2qox|2qox]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qow OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qow RCSB], [http://www.ebi.ac.uk/pdbsum/2qow PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/2qow_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The widely used antibiotic spectinomycin inhibits bacterial protein synthesis by blocking translocation of messenger RNA and transfer RNAs on the ribosome. Here, we show that in crystals of the Escherichia coli 70S ribosome spectinomycin binding traps a distinct swiveling state of the head domain of the small ribosomal subunit. Spectinomycin also alters the rate and completeness of reverse translocation in vitro. These structural and biochemical data indicate that in solution spectinomycin sterically blocks swiveling of the head domain of the small ribosomal subunit and thereby disrupts the translocation cycle.
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{{STRUCTURE_2qow| PDB=2qow | SCENE= }}
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A steric block in translation caused by the antibiotic spectinomycin.,Borovinskaya MA, Shoji S, Holton JM, Fredrick K, Cate JH ACS Chem Biol. 2007 Aug 17;2(8):545-52. Epub 2007 Aug 10. PMID:17696316<ref>PMID:17696316</ref>
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===Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_17696316}}
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==About this Structure==
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[[2qow]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QOW OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein S10|Ribosomal protein S10]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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*[[Ribosomal protein S11|Ribosomal protein S11]]
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== References ==
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*[[Ribosomal protein S12|Ribosomal protein S12]]
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<references/>
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*[[Ribosomal protein S13|Ribosomal protein S13]]
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__TOC__
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*[[Ribosomal protein S14|Ribosomal protein S14]]
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</StructureSection>
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*[[Ribosomal protein S15|Ribosomal protein S15]]
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*[[Ribosomal protein S16|Ribosomal protein S16]]
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*[[Ribosomal protein S17|Ribosomal protein S17]]
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*[[Ribosomal protein S18|Ribosomal protein S18]]
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*[[Ribosomal protein S19|Ribosomal protein S19]]
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*[[Ribosomal protein S2|Ribosomal protein S2]]
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*[[Ribosomal protein S20|Ribosomal protein S20]]
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*[[Ribosomal protein S21|Ribosomal protein S21]]
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*[[Ribosomal protein S3|Ribosomal protein S3]]
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*[[Ribosomal protein S4|Ribosomal protein S4]]
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*[[Ribosomal protein S5|Ribosomal protein S5]]
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*[[Ribosomal protein S6|Ribosomal protein S6]]
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*[[Ribosomal protein S7|Ribosomal protein S7]]
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*[[Ribosomal protein S8|Ribosomal protein S8]]
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*[[Ribosomal protein S9|Ribosomal protein S9]]
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==Reference==
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<ref group="xtra">PMID:017696316</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Borovinskaya, M A.]]
[[Category: Borovinskaya, M A.]]

Revision as of 08:39, 29 September 2014

Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin. This file contains the 30S subunit of the second 70S ribosome, with spectinomycin bound. The entire crystal structure contains two 70S ribosomes.

2qow, resolution 3.93Å

Drag the structure with the mouse to rotate

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OCA

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