1gk8
From Proteopedia
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| - | [[Image:1gk8.gif|left|200px]] | + | [[Image:1gk8.gif|left|200px]] |
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| - | '''RUBISCO FROM CHLAMYDOMONAS REINHARDTII''' | + | {{Structure |
| + | |PDB= 1gk8 |SIZE=350|CAPTION= <scene name='initialview01'>1gk8</scene>, resolution 1.4Å | ||
| + | |SITE= <scene name='pdbsite=MGA:Active+Site+Residues+Coordinating+Mg'>MGA</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''RUBISCO FROM CHLAMYDOMONAS REINHARDTII''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GK8 is a [ | + | 1GK8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GK8 OCA]. |
==Reference== | ==Reference== | ||
| - | First crystal structure of Rubisco from a green alga, Chlamydomonas reinhardtii., Taylor TC, Backlund A, Bjorhall K, Spreitzer RJ, Andersson I, J Biol Chem. 2001 Dec 21;276(51):48159-64. Epub 2001 Oct 18. PMID:[http:// | + | First crystal structure of Rubisco from a green alga, Chlamydomonas reinhardtii., Taylor TC, Backlund A, Bjorhall K, Spreitzer RJ, Andersson I, J Biol Chem. 2001 Dec 21;276(51):48159-64. Epub 2001 Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11641402 11641402] |
[[Category: Chlamydomonas reinhardtii]] | [[Category: Chlamydomonas reinhardtii]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: rubisco]] | [[Category: rubisco]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:24:18 2008'' |
Revision as of 09:24, 20 March 2008
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| , resolution 1.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Ribulose-bisphosphate carboxylase, with EC number 4.1.1.39 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RUBISCO FROM CHLAMYDOMONAS REINHARDTII
Overview
The crystal structure of Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase) from the unicellular green alga Chlamydomonas reinhardtii has been determined to 1.4 A resolution. Overall, the structure shows high similarity to the previously determined structures of L8S8 Rubisco enzymes. The largest difference is found in the loop between beta strands A and B of the small subunit (betaA-betaB loop), which is longer by six amino acid residues than the corresponding region in Rubisco from Spinacia. Mutations of residues in the betaA-betaB loop have been shown to affect holoenzyme stability and catalytic properties. The information contained in the Chlamydomonas structure enables a more reliable analysis of the effect of these mutations. No electron density was observed for the last 13 residues of the small subunit, which are assumed to be disordered in the crystal. Because of the high resolution of the data, some posttranslational modifications are unambiguously apparent in the structure. These include cysteine and N-terminal methylations and proline 4-hydroxylations.
About this Structure
1GK8 is a Protein complex structure of sequences from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.
Reference
First crystal structure of Rubisco from a green alga, Chlamydomonas reinhardtii., Taylor TC, Backlund A, Bjorhall K, Spreitzer RJ, Andersson I, J Biol Chem. 2001 Dec 21;276(51):48159-64. Epub 2001 Oct 18. PMID:11641402
Page seeded by OCA on Thu Mar 20 11:24:18 2008
