3ixz

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[[Image:3ixz.png|left|200px]]
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==Pig gastric H+/K+-ATPase complexed with aluminium fluoride==
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<StructureSection load='3ixz' size='340' side='right' caption='[[3ixz]], [[Resolution|resolution]] 6.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ixz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. The October 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sodium-Potassium Pump'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_10 10.2210/rcsb_pdb/mom_2009_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IXZ FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3b8e|3b8e]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ixz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ixz RCSB], [http://www.ebi.ac.uk/pdbsum/3ixz PDBsum]</span></td></tr>
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<table>
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== Disease ==
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[[http://www.uniprot.org/uniprot/ATP4B_PIG ATP4B_PIG]] Note=Parietal cell autoantigen associated with autoimmune gastritis.
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== Function ==
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[[http://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/3ixz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The gastric H(+),K(+)-ATPase is an ATP-driven proton pump responsible for generating a million-fold proton gradient across the gastric membrane. We present the structure of gastric H(+),K(+)-ATPase at 6.5 A resolution as determined by electron crystallography of two-dimensional crystals. The structure shows the catalytic alpha-subunit and the non-catalytic beta-subunit in a pseudo-E(2)P conformation. Different from Na(+),K(+)-ATPase, the N-terminal tail of the beta-subunit is in direct contact with the phosphorylation domain of the alpha-subunit. This interaction may hold the phosphorylation domain in place, thus stabilizing the enzyme conformation and preventing the reverse reaction of the transport cycle. Indeed, truncation of the beta-subunit N-terminus allowed the reverse reaction to occur. These results suggest that the beta-subunit N-terminus prevents the reverse reaction from E(2)P to E(1)P, which is likely to be relevant for the generation of a large H(+) gradient in vivo situation.
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{{STRUCTURE_3ixz| PDB=3ixz | SCENE= }}
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Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle.,Abe K, Tani K, Nishizawa T, Fujiyoshi Y EMBO J. 2009 Jun 3;28(11):1637-43. Epub 2009 Apr 23. PMID:19387495<ref>PMID:19387495</ref>
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===Pig gastric H+/K+-ATPase complexed with aluminium fluoride===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19387495}}
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==About this Structure==
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[[3ixz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. The October 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sodium-Potassium Pump'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_10 10.2210/rcsb_pdb/mom_2009_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXZ OCA].
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==See Also==
==See Also==
*[[ATPase|ATPase]]
*[[ATPase|ATPase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019387495</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Hydrogen/potassium-exchanging ATPase]]
[[Category: Hydrogen/potassium-exchanging ATPase]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]

Revision as of 11:00, 29 September 2014

Pig gastric H+/K+-ATPase complexed with aluminium fluoride

3ixz, resolution 6.50Å

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