1go7

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[[Image:1go7.jpg|left|200px]]<br /><applet load="1go7" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1go7.jpg|left|200px]]
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caption="1go7, resolution 2.10&Aring;" />
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'''THE METZINCIN'S METHIONINE: PRTC M226C-E189K DOUBLE MUTANT'''<br />
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{{Structure
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|PDB= 1go7 |SIZE=350|CAPTION= <scene name='initialview01'>1go7</scene>, resolution 2.10&Aring;
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|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+P'>AC1</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''THE METZINCIN'S METHIONINE: PRTC M226C-E189K DOUBLE MUTANT'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1GO7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+P'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GO7 OCA].
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1GO7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GO7 OCA].
==Reference==
==Reference==
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Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11445573 11445573]
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Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11445573 11445573]
[[Category: Erwinia chrysanthemi]]
[[Category: Erwinia chrysanthemi]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: protease]]
[[Category: protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:52:11 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:25:45 2008''

Revision as of 09:25, 20 March 2008

Image:1go7.jpg


PDB ID 1go7

Drag the structure with the mouse to rotate
, resolution 2.10Å
Sites:
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



THE METZINCIN'S METHIONINE: PRTC M226C-E189K DOUBLE MUTANT


Overview

Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes.

About this Structure

1GO7 is a Single protein structure of sequence from Erwinia chrysanthemi. Full crystallographic information is available from OCA.

Reference

Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:11445573

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