3hi8

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[[Image:3hi8.png|left|200px]]
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==Crystal structure of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii==
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<StructureSection load='3hi8' size='340' side='right' caption='[[3hi8]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hi8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Haloferax_volcanii Haloferax volcanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3HI8 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HVO_0175 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2246 Haloferax volcanii])</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3hi8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hi8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3hi8 RCSB], [http://www.ebi.ac.uk/pdbsum/3hi8 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hi/3hi8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The sliding clamp proliferating cell nuclear antigen (PCNA) plays vital roles in many aspects of DNA replication and repair in eukaryotic cells and in archaea. Realising the full potential of archaea as a model for PCNA function requires a combination of biochemical and genetic approaches. In order to provide a platform for subsequent reverse genetic analysis, PCNA from the halophilic archaeon Haloferax volcanii was subjected to crystallographic analysis. The gene was cloned and expressed in Escherichia coli and the protein was purified by affinity chromatography and crystallized by the vapour-diffusion technique. The structure was determined by molecular replacement and refined at 3.5 A resolution to a final R factor of 23.7% (R(free) = 25%). PCNA from H. volcanii was found to be homotrimeric and to resemble other homotrimeric PCNA clamps but with several differences that appear to be associated with adaptation of the protein to the high intracellular salt concentrations found in H. volcanii cells.
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{{STRUCTURE_3hi8| PDB=3hi8 | SCENE= }}
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Structural insights into the adaptation of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii to a high-salt environment.,Morgunova E, Gray FC, Macneill SA, Ladenstein R Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1081-8. Epub 2009, Sep 16. PMID:19770505<ref>PMID:19770505</ref>
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===Crystal structure of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19770505}}
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==About this Structure==
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[[3hi8]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Haloferax_volcanii Haloferax volcanii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HI8 OCA].
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==See Also==
==See Also==
*[[Proliferating Cell Nuclear Antigen|Proliferating Cell Nuclear Antigen]]
*[[Proliferating Cell Nuclear Antigen|Proliferating Cell Nuclear Antigen]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019770505</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Haloferax volcanii]]
[[Category: Haloferax volcanii]]
[[Category: Gray, F C.]]
[[Category: Gray, F C.]]

Revision as of 13:16, 29 September 2014

Crystal structure of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii

3hi8, resolution 3.20Å

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