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3eq4

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[[Image:3eq4.png|left|200px]]
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==Model of tRNA(Leu)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM==
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<StructureSection load='3eq4' size='340' side='right' caption='[[3eq4]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3eq4]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EQ4 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2avy|2avy]], [[2aw4|2aw4]], [[1qza|1qza]], [[1ob2|1ob2]], [[3ep2|3ep2]], [[3eq3|3eq3]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3eq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3eq4 RCSB], [http://www.ebi.ac.uk/pdbsum/3eq4 PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eq/3eq4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of the kirromycin-stalled ribosome bound with ternary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI). The three maps suggest a common binding manner of cognate aa-tRNAs in their specific binding with both the ribosome and EF-Tu. All three aa-tRNAs have the same 'loaded spring' conformation with a kink and twist between the D-stem and anticodon stem. The three complexes are similarly integrated in an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-binding CCA end of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the accuracy of aa-tRNA selection.
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{{STRUCTURE_3eq4| PDB=3eq4 | SCENE= }}
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Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM.,Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J EMBO J. 2008 Dec 17;27(24):3322-31. Epub 2008 Nov 20. PMID:19020518<ref>PMID:19020518</ref>
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===Model of tRNA(Leu)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19020518}}
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==About this Structure==
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[[3eq4]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQ4 OCA].
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==See Also==
==See Also==
*[[Elongation factor|Elongation factor]]
*[[Elongation factor|Elongation factor]]
*[[Ribosomal protein L11|Ribosomal protein L11]]
*[[Ribosomal protein L11|Ribosomal protein L11]]
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*[[Ribosomal protein S12|Ribosomal protein S12]]
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:019020518</ref><references group="xtra"/>
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</StructureSection>
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli k-12]]
[[Category: Agirrezabala, X.]]
[[Category: Agirrezabala, X.]]

Revision as of 13:22, 29 September 2014

Model of tRNA(Leu)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM

3eq4, resolution 12.00Å

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