1gua
From Proteopedia
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| - | [[Image:1gua.gif|left|200px]] | + | [[Image:1gua.gif|left|200px]] |
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| - | '''HUMAN RAP1A, RESIDUES 1-167, DOUBLE MUTANT (E30D,K31E) COMPLEXED WITH GPPNHP AND THE RAS-BINDING-DOMAIN OF HUMAN C-RAF1, RESIDUES 51-131''' | + | {{Structure |
| + | |PDB= 1gua |SIZE=350|CAPTION= <scene name='initialview01'>1gua</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER'>GNP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= HUMAN C-RAF1 GENE RESIDUES 51 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''HUMAN RAP1A, RESIDUES 1-167, DOUBLE MUTANT (E30D,K31E) COMPLEXED WITH GPPNHP AND THE RAS-BINDING-DOMAIN OF HUMAN C-RAF1, RESIDUES 51-131''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GUA is a [ | + | 1GUA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUA OCA]. |
==Reference== | ==Reference== | ||
| - | Ras/Rap effector specificity determined by charge reversal., Nassar N, Horn G, Herrmann C, Block C, Janknecht R, Wittinghofer A, Nat Struct Biol. 1996 Aug;3(8):723-9. PMID:[http:// | + | Ras/Rap effector specificity determined by charge reversal., Nassar N, Horn G, Herrmann C, Block C, Janknecht R, Wittinghofer A, Nat Struct Biol. 1996 Aug;3(8):723-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8756332 8756332] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: oncogene protein/kinase/effector protein gtp-binding-protein]] | [[Category: oncogene protein/kinase/effector protein gtp-binding-protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:27:59 2008'' |
Revision as of 09:28, 20 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | HUMAN C-RAF1 GENE RESIDUES 51 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN RAP1A, RESIDUES 1-167, DOUBLE MUTANT (E30D,K31E) COMPLEXED WITH GPPNHP AND THE RAS-BINDING-DOMAIN OF HUMAN C-RAF1, RESIDUES 51-131
Contents |
Overview
Members of the Ras subfamily of small GTP-binding proteins have been shown to be promiscuous towards a variety of putative effector molecules such as the protein kinase c-Raf and the Ral-specific guanine nucleotide exchange factor (Ral-GEF). To address the question of specificity of interactions we have introduced the mutations E30D and K31E into Rap and show biochemically, by X-ray structure analysis and by transfection in vivo that the identical core effector region of Ras and Rap (residues 32-40) is responsible for molecular recognition, but that residues outside this region are responsible for the specificity of the interaction. The major determinant for the switch in specificity is the opposite charge of residue 31--Lys in Rap, Glu in Ras--which creates a favourable complementary interface for the Ras-Raf interaction.
Disease
Known diseases associated with this structure: LEOPARD syndrome 2 OMIM:[164760], Noonan syndrome 5 OMIM:[164760]
About this Structure
1GUA is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Ras/Rap effector specificity determined by charge reversal., Nassar N, Horn G, Herrmann C, Block C, Janknecht R, Wittinghofer A, Nat Struct Biol. 1996 Aug;3(8):723-9. PMID:8756332
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