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2j6h

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[[Category: transferase]]
[[Category: transferase]]
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Revision as of 15:20, 30 October 2007

Image:2j6h.gif

2j6h, resolution 2.35Å

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E. COLI GLUCOSAMINE-6-P SYNTHASE IN COMPLEX WITH GLUCOSE-6P AND 5-OXO-L-NORLEUCINE

Overview

Glucosamine-6P synthase catalyzes the synthesis of glucosamine-6P from, fructose-6P and glutamine and uses a channel to transfer ammonia from its, glutaminase to its synthase active site. X-ray structures of, glucosamine-6P synthase have been determined at 2.05 Angstroms resolution, in the presence of fructose-6P and at 2.35 Angstroms resolution in the, presence of fructose-6P and 6-diazo-5-oxo-L-norleucine, a glutamine, affinity analog that covalently modifies the N-terminal catalytic, cysteine, therefore mimicking the gamma-glutamyl-thioester intermediate, formed during hydrolysis of glutamine. The fixation of the glutamine, analog activates the enzyme through several major structural changes: 1), the closure of a loop to shield the glutaminase site accompanied by, significant domain ... [(full description)]

About this Structure

2J6H is a [Single protein] structure of sequence from [Escherichia coli] with G6Q and ONL as [ligands]. This structure superseeds the now removed PDB entry 2BPJ. Active as [Glutamine--fructose-6-phosphate transaminase (isomerizing)], with EC number [2.6.1.16]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase., Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B, J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9. PMID:16339762

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