1gw9
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1gw9.gif|left|200px]] | + | [[Image:1gw9.gif|left|200px]] |
| - | + | ||
| - | '''TRI-IODIDE DERIVATIVE OF XYLOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS''' | + | {{Structure |
| + | |PDB= 1gw9 |SIZE=350|CAPTION= <scene name='initialview01'>1gw9</scene>, resolution 1.55Å | ||
| + | |SITE= <scene name='pdbsite=CA1:Lxc+Binding+Site+For+Chain+A'>CA1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=LXC:BETA-L-XYLOPYRANOSE'>LXC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=IOD:IODIDE ION'>IOD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''TRI-IODIDE DERIVATIVE OF XYLOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1GW9 is a [ | + | 1GW9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_rubiginosus Streptomyces rubiginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GW9 OCA]. |
==Reference== | ==Reference== | ||
| - | Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:[http:// | + | Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12037300 12037300] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces rubiginosus]] | [[Category: Streptomyces rubiginosus]] | ||
| Line 24: | Line 33: | ||
[[Category: xylose metabolism]] | [[Category: xylose metabolism]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:28:47 2008'' |
Revision as of 09:28, 20 March 2008
| |||||||
| , resolution 1.55Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Xylose isomerase, with EC number 5.3.1.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TRI-IODIDE DERIVATIVE OF XYLOSE ISOMERASE FROM STREPTOMYCES RUBIGINOSUS
Overview
A series of experiments performed at Cu Kalpha wavelength on in-house X-ray equipment are presented which investigate two possibilities for enhancing the experimental phasing signal by means of (i) triiodide/iodide soaks using KI/I(2) and (ii) combinations of counter-ions introduced using the short cryosoak method. Triiodide-derivative crystal structures for five test proteins have been refined and reveal that iodine can bind as polyiodide and single iodide ions through hydrophobic and hydrogen-bonding interactions both at the molecular surface and in intramolecular and intermolecular cavities. In three cases, the structures could be automatically determined with autoSHARP using in-house SAD and SIRAS data. The investigation of combinatorial counter-ion replacement using multiple salts with Na(+) and Cs(+) as cations and I(-) and Cl(-) as anions reveals that, for the case of hen egg-white lysozyme, significant improvement in phasing signal is obtained by the combined use of salts compared with SIRAS methods using native and single short-soak derivative data sets.
About this Structure
1GW9 is a Single protein structure of sequence from Streptomyces rubiginosus. Full crystallographic information is available from OCA.
Reference
Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:12037300
Page seeded by OCA on Thu Mar 20 11:28:47 2008
