1gwl
From Proteopedia
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| - | [[Image:1gwl.jpg|left|200px]] | + | [[Image:1gwl.jpg|left|200px]] |
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| - | '''CARBOHYDRATE BINDING MODULE FAMILY29 COMPLEXED WITH MANNOHEXAOSE''' | + | {{Structure |
| + | |PDB= 1gwl |SIZE=350|CAPTION= <scene name='initialview01'>1gwl</scene>, resolution 1.51Å | ||
| + | |SITE= <scene name='pdbsite=BM1:Bma+Binding+Site+For+Chain+A+Residue+A1148'>BM1</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CARBOHYDRATE BINDING MODULE FAMILY29 COMPLEXED WITH MANNOHEXAOSE''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GWL is a [ | + | 1GWL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Piromyces_equi Piromyces equi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWL OCA]. |
==Reference== | ==Reference== | ||
| - | Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:[http:// | + | Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12391332 12391332] |
[[Category: Piromyces equi]] | [[Category: Piromyces equi]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: mannohexaose]] | [[Category: mannohexaose]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:29:02 2008'' |
Revision as of 09:29, 20 March 2008
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| , resolution 1.51Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CARBOHYDRATE BINDING MODULE FAMILY29 COMPLEXED WITH MANNOHEXAOSE
Overview
Carbohydrate-protein recognition is central to many biological processes. Enzymes that act on polysaccharide substrates frequently contain noncatalytic domains, "carbohydrate-binding modules" (CBMs), that target the enzyme to the appropriate substrate. CBMs that recognize specific plant structural polysaccharides are often able to accommodate both the variable backbone and the side-chain decorations of heterogeneous ligands. "CBM29" modules, derived from a noncatalytic component of the Piromyces equi cellulase/hemicellulase complex, provide an example of this selective yet flexible recognition. They discriminate strongly against some polysaccharides while remaining relatively promiscuous toward both beta-1,4-linked manno- and cello-oligosaccharides. This feature may reflect preferential, but flexible, targeting toward glucomannans in the plant cell wall. The three-dimensional structure of CBM29-2 and its complexes with cello- and mannohexaose reveal a beta-jelly-roll topology, with an extended binding groove on the concave surface. The orientation of the aromatic residues complements the conformation of the target sugar polymer while accommodation of both manno- and gluco-configured oligo- and polysaccharides is conferred by virtue of the plasticity of the direct interactions from their axial and equatorial 2-hydroxyls, respectively. Such flexible ligand recognition targets the anaerobic fungal complex to a range of different components in the plant cell wall and thus plays a pivotal role in the highly efficient degradation of this composite structure by the microbial eukaryote.
About this Structure
1GWL is a Single protein structure of sequence from Piromyces equi. Full crystallographic information is available from OCA.
Reference
Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:12391332
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