1gxw

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[[Image:1gxw.gif|left|200px]]<br /><applet load="1gxw" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1gxw.gif|left|200px]]
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caption="1gxw, resolution 2.18&Aring;" />
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'''THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE'''<br />
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{{Structure
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|PDB= 1gxw |SIZE=350|CAPTION= <scene name='initialview01'>1gxw</scene>, resolution 2.18&Aring;
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|SITE= <scene name='pdbsite=CA1:LYS+Binding+Site+For+Chain+A'>CA1</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene> and <scene name='pdbligand=VAL:VALINE'>VAL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27]
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|GENE=
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}}
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'''THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1GXW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SCN:'>SCN</scene> and <scene name='pdbligand=VAL:'>VAL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] Known structural/functional Site: <scene name='pdbsite=CA1:LYS+Binding+Site+For+Chain+A'>CA1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXW OCA].
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1GXW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXW OCA].
==Reference==
==Reference==
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The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate., Gaucher JF, Selkti M, Prange T, Tomas A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12454500 12454500]
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The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate., Gaucher JF, Selkti M, Prange T, Tomas A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12454500 12454500]
[[Category: Bacillus thermoproteolyticus]]
[[Category: Bacillus thermoproteolyticus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: thiocyanate]]
[[Category: thiocyanate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:29:27 2008''

Revision as of 09:29, 20 March 2008

Image:1gxw.gif


PDB ID 1gxw

Drag the structure with the mouse to rotate
, resolution 2.18Å
Sites:
Ligands: , , and
Activity: Thermolysin, with EC number 3.4.24.27
Coordinates: save as pdb, mmCIF, xml



THE 2.2 A RESOLUTION STRUCTURE OF THERMOLYSIN CRYSTALLIZED IN PRESENCE OF POTASSIUM THIOCYANATE


Overview

A new crystallization protocol for thermolysin (EC 3.4.24.27) from Bacillus thermoproteolyticus is presented. After dissolving the protein in the presence of KSCN, which avoids the use of DMSO and CsCl, crystals were obtained following the salting-in method. Crystal cell parameters are isomorphous with those previously reported from DMSO/CsCl mixtures. The new SCN(-) crystal structure has been analyzed. It shows the presence of one thiocyanate ion in the catalytic site and several rearrangements in the S(1) and S(2) subsites. These results are in agreement with the measurements of Inouye et al. [(1998), J. Biochem. (Tokyo), 123, 847-852], who observed in solution that the solubility of TLN, which is particularly poor in low ionic strength solutions, increases dramatically in the presence of several neutral salts. The results reported here suggest possible explanations for the solubility increase and for the inhibitory effects of high SCN(-) concentrations on thermolysin activity.

About this Structure

1GXW is a Single protein structure of sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA.

Reference

The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate., Gaucher JF, Selkti M, Prange T, Tomas A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:12454500

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