1gzc

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[[Image:1gzc.jpg|left|200px]]<br /><applet load="1gzc" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1gzc.jpg|left|200px]]
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caption="1gzc, resolution 1.58&Aring;" />
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'''HIGH-RESOLUTION CRYSTAL STRUCTURE OF ERYTHRINA CRISTAGALLI LECTIN IN COMPLEX WITH LACTOSE'''<br />
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{{Structure
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|PDB= 1gzc |SIZE=350|CAPTION= <scene name='initialview01'>1gzc</scene>, resolution 1.58&Aring;
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|SITE= <scene name='pdbsite=MNA:Lat+Binding+Site+For+Chain+A'>MNA</scene>
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|LIGAND= <scene name='pdbligand=LAT:LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''HIGH-RESOLUTION CRYSTAL STRUCTURE OF ERYTHRINA CRISTAGALLI LECTIN IN COMPLEX WITH LACTOSE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1GZC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota] with <scene name='pdbligand=LAT:'>LAT</scene>, <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=MNA:Lat+Binding+Site+For+Chain+A'>MNA</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZC OCA].
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1GZC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZC OCA].
==Reference==
==Reference==
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High-resolution crystal structures of Erythrina cristagalli lectin in complex with lactose and 2'-alpha-L-fucosyllactose and correlation with thermodynamic binding data., Svensson C, Teneberg S, Nilsson CL, Kjellberg A, Schwarz FP, Sharon N, Krengel U, J Mol Biol. 2002 Aug 2;321(1):69-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12139934 12139934]
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High-resolution crystal structures of Erythrina cristagalli lectin in complex with lactose and 2'-alpha-L-fucosyllactose and correlation with thermodynamic binding data., Svensson C, Teneberg S, Nilsson CL, Kjellberg A, Schwarz FP, Sharon N, Krengel U, J Mol Biol. 2002 Aug 2;321(1):69-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12139934 12139934]
[[Category: Eukaryota]]
[[Category: Eukaryota]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: legume lectin]]
[[Category: legume lectin]]
[[Category: protein]]
[[Category: protein]]
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[[Category: protein-carbohydrate interactions]]
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[[Category: protein-carbohydrate interaction]]
[[Category: saccharide]]
[[Category: saccharide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:30:04 2008''

Revision as of 09:30, 20 March 2008

Image:1gzc.jpg


PDB ID 1gzc

Drag the structure with the mouse to rotate
, resolution 1.58Å
Sites:
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



HIGH-RESOLUTION CRYSTAL STRUCTURE OF ERYTHRINA CRISTAGALLI LECTIN IN COMPLEX WITH LACTOSE


Overview

The primary sequence of Erythrina cristagalli lectin (ECL) was mapped by mass spectrometry, and the crystal structures of the lectin in complex with lactose and 2'-alpha-L-fucosyllactose were determined at 1.6A and 1.7A resolution, respectively. The two complexes were compared with the crystal structure of the closely related Erythrina corallodendron lectin (ECorL) in complex with lactose, with the crystal structure of the Ulex europaeus lectin II in complex with 2'-alpha-L-fucosyllactose, and with two modeled complexes of ECorL with 2'-alpha-L-fucosyl-N-acetyllactosamine. The molecular models are very similar to the crystal structure of ECL in complex with 2'-alpha-L-fucosyllactose with respect to the overall mode of binding, with the L-fucose fitting snugly into the cavity surrounded by Tyr106, Tyr108, Trp135 and Pro134 adjoining the primary combining site of the lectin. Marked differences were however noted between the models and the experimental structure in the network of hydrogen bonds and hydrophobic interactions holding the L-fucose in the combining site of the lectin, pointing to limitations of the modeling approach. In addition to the structural characterization of the ECL complexes, an effort was undertaken to correlate the structural data with thermodynamic data obtained from microcalorimetry, revealing the importance of the water network in the lectin combining site for carbohydrate binding.

About this Structure

1GZC is a Single protein structure of sequence from Eukaryota. Full crystallographic information is available from OCA.

Reference

High-resolution crystal structures of Erythrina cristagalli lectin in complex with lactose and 2'-alpha-L-fucosyllactose and correlation with thermodynamic binding data., Svensson C, Teneberg S, Nilsson CL, Kjellberg A, Schwarz FP, Sharon N, Krengel U, J Mol Biol. 2002 Aug 2;321(1):69-83. PMID:12139934

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