1h18

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[[Image:1h18.jpg|left|200px]]<br /><applet load="1h18" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1h18.jpg|left|200px]]
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caption="1h18, resolution 2.3&Aring;" />
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'''PYRUVATE FORMATE-LYASE (E.COLI) IN COMPLEX WITH PYRUVATE'''<br />
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{{Structure
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|PDB= 1h18 |SIZE=350|CAPTION= <scene name='initialview01'>1h18</scene>, resolution 2.3&Aring;
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|SITE= <scene name='pdbsite=418:Pg4+Binding+Site+For+Chain+B'>418</scene>
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene>, <scene name='pdbligand=DTL:D-TREITOL'>DTL</scene> and <scene name='pdbligand=PG4:TETRAETHYLENE GLYCOL'>PG4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Formate_C-acetyltransferase Formate C-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.54 2.3.1.54]
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|GENE=
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}}
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'''PYRUVATE FORMATE-LYASE (E.COLI) IN COMPLEX WITH PYRUVATE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1H18 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=PYR:'>PYR</scene>, <scene name='pdbligand=DTL:'>DTL</scene> and <scene name='pdbligand=PG4:'>PG4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_C-acetyltransferase Formate C-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.54 2.3.1.54] Known structural/functional Site: <scene name='pdbsite=418:Pg4+Binding+Site+For+Chain+B'>418</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H18 OCA].
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1H18 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H18 OCA].
==Reference==
==Reference==
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X-ray structure of pyruvate formate-lyase in complex with pyruvate and CoA. How the enzyme uses the Cys-418 thiyl radical for pyruvate cleavage., Becker A, Kabsch W, J Biol Chem. 2002 Oct 18;277(42):40036-42. Epub 2002 Aug 5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12163496 12163496]
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X-ray structure of pyruvate formate-lyase in complex with pyruvate and CoA. How the enzyme uses the Cys-418 thiyl radical for pyruvate cleavage., Becker A, Kabsch W, J Biol Chem. 2002 Oct 18;277(42):40036-42. Epub 2002 Aug 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12163496 12163496]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Formate C-acetyltransferase]]
[[Category: Formate C-acetyltransferase]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:30:49 2008''

Revision as of 09:30, 20 March 2008

Image:1h18.jpg


PDB ID 1h18

Drag the structure with the mouse to rotate
, resolution 2.3Å
Sites:
Ligands: , , and
Activity: Formate C-acetyltransferase, with EC number 2.3.1.54
Coordinates: save as pdb, mmCIF, xml



PYRUVATE FORMATE-LYASE (E.COLI) IN COMPLEX WITH PYRUVATE


Overview

The glycyl radical enzyme pyruvate formate-lyase (PFL) synthesizes acetyl-CoA and formate from pyruvate and CoA. With the crystal structure of the non-radical form of PFL in complex with its two substrates, we have trapped the moment prior to pyruvate cleavage. The structure reveals how the active site aligns the scissile bond of pyruvate for radical attack, prevents non-radical side reactions of the pyruvate, and confines radical migration. The structure shows CoA in a syn conformation awaiting pyruvate cleavage. By changing to an anti conformation, without affecting the adenine binding mode of CoA, the thiol of CoA could pick up the acetyl group resulting from pyruvate cleavage.

About this Structure

1H18 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

X-ray structure of pyruvate formate-lyase in complex with pyruvate and CoA. How the enzyme uses the Cys-418 thiyl radical for pyruvate cleavage., Becker A, Kabsch W, J Biol Chem. 2002 Oct 18;277(42):40036-42. Epub 2002 Aug 5. PMID:12163496

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