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1h1l

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[[Image:1h1l.jpg|left|200px]]<br /><applet load="1h1l" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1h1l.jpg|left|200px]]
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caption="1h1l, resolution 1.90&Aring;" />
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'''NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT'''<br />
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{{Structure
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|PDB= 1h1l |SIZE=350|CAPTION= <scene name='initialview01'>1h1l</scene>, resolution 1.90&Aring;
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|SITE= <scene name='pdbsite=CT1:Clf+Binding+Site+For+Chain+D'>CT1</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CFM:FE-MO-S+CLUSTER'>CFM</scene> and <scene name='pdbligand=CLF:FE(8)-S(7) CLUSTER'>CLF</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1]
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|GENE=
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}}
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'''NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1H1L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CIT:'>CIT</scene>, <scene name='pdbligand=CFM:'>CFM</scene> and <scene name='pdbligand=CLF:'>CLF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1] Known structural/functional Site: <scene name='pdbsite=CT1:Clf+Binding+Site+For+Chain+D'>CT1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H1L OCA].
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1H1L is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H1L OCA].
==Reference==
==Reference==
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Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco)., Mayer SM, Gormal CA, Smith BE, Lawson DM, J Biol Chem. 2002 Sep 20;277(38):35263-6. Epub 2002 Jul 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12133839 12133839]
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Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco)., Mayer SM, Gormal CA, Smith BE, Lawson DM, J Biol Chem. 2002 Sep 20;277(38):35263-6. Epub 2002 Jul 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12133839 12133839]
[[Category: Klebsiella pneumoniae]]
[[Category: Klebsiella pneumoniae]]
[[Category: Nitrogenase]]
[[Category: Nitrogenase]]
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[[Category: biological nitrogen fixation]]
[[Category: biological nitrogen fixation]]
[[Category: electron transfer]]
[[Category: electron transfer]]
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[[Category: molybdoenzymes]]
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[[Category: molybdoenzyme]]
[[Category: nitrogen metabolism]]
[[Category: nitrogen metabolism]]
[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:56:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:30:54 2008''

Revision as of 09:30, 20 March 2008

Image:1h1l.jpg


PDB ID 1h1l

Drag the structure with the mouse to rotate
, resolution 1.90Å
Sites:
Ligands: , , , and
Activity: Nitrogenase, with EC number 1.18.6.1
Coordinates: save as pdb, mmCIF, xml



NITROGENASE MO-FE PROTEIN FROM KLEBSIELLA PNEUMONIAE, NIFV MUTANT


Overview

The x-ray crystal structure of NifV(-) Klebsiella pneumoniae nitrogenase MoFe protein (NifV(-) Kp1) has been determined and refined to a resolution of 1.9 A. This is the first structure for a nitrogenase MoFe protein with an altered cofactor. Moreover, it is the first direct evidence that the organic acid citrate is not just present, but replaces homocitrate as a ligand to the molybdenum atom of the iron molybdenum cofactor (FeMoco). Subsequent refinement of the structure revealed that the citrate was present at reduced occupancy.

About this Structure

1H1L is a Protein complex structure of sequences from Klebsiella pneumoniae. Full crystallographic information is available from OCA.

Reference

Crystallographic analysis of the MoFe protein of nitrogenase from a nifV mutant of Klebsiella pneumoniae identifies citrate as a ligand to the molybdenum of iron molybdenum cofactor (FeMoco)., Mayer SM, Gormal CA, Smith BE, Lawson DM, J Biol Chem. 2002 Sep 20;277(38):35263-6. Epub 2002 Jul 19. PMID:12133839

Page seeded by OCA on Thu Mar 20 11:30:54 2008

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